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August 2014; 1 (2) ArticleOpen Access

CSF isoprostane levels are a biomarker of oxidative stress in multiple sclerosis

Fozia Mir, Donald Lee, Hetal Ray, Saud A. Sadiq
First published August 14, 2014, DOI: https://doi.org/10.1212/NXI.0000000000000021
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Abstract

Objective: To investigate the potential of 8-iso-prostaglandin F2α (8-iso-PGF2α) as a biomarker for disease activity and oxidative stress in the CSF of patients with multiple sclerosis (MS).

Methods: The isoprostane 8-iso-PGF2α is an established biomarker for in vivo oxidative stress and lipid peroxidation. We measured CSF 8-isoPGF2α levels in 231 patients with MS (74 with relapsing-remitting MS, 67 with primary progressive MS, and 90 with secondary progressive MS [SPMS]) and 40 controls using a competition ELISA.

Results: We found increased CSF levels of 8-iso-PGF2α in patients with MS compared to controls, with the most striking values in a subgroup of patients with SPMS. Furthermore, the increase in 8-iso-PGF2α correlated with other parameters of lipid peroxidation as well as with a decrease in the total antioxidant status in the MS CSF samples.

Conclusions: Our study demonstrates that CSF levels of 8-iso-PGF2α may serve as a biomarker of oxidative stress in MS. Further investigation will help establish the pathologic and clinical significance of our preliminary findings.

GLOSSARY

8-iso-PGF2α=
8-iso-prostaglandin F2α;
AAPH=
2,2′-azobis-2-methyl-propanimidamide dihydrochloride;
DMF=
dimethyl fumarate;
EAE=
experimental allergic encephalitis;
EDSS=
Expanded Disability Status Scale;
GSSG=
oxidized glutathione;
IMSMP=
International Multiple Sclerosis Management Practice;
IRB=
institutional review board;
MDA=
malondialdehyde;
MS=
multiple sclerosis;
OND=
other neurologic disorder;
PPMS=
primary progressive MS;
ROS=
reactive oxygen species;
RRMS=
relapsing-remitting MS;
SOD=
superoxide dismutase;
SPMS=
secondary progressive MS;
TAS=
total antioxidant status;
TBARS=
thiobarbituric acid reactive substances

Footnotes

  • Go to Neurology.org/nn for full disclosures. Funding information and disclosures deemed relevant by the authors, if any, are provided at the end of the article. The Article Processing Charge was paid by the TISCH MS Research Center of NYT.

  • Received January 28, 2014.
  • Accepted in final form June 27, 2014.

This is an open access article distributed under the terms of the Creative Commons Attribution-Noncommercial No Derivative 3.0 License, which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially.

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