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January 2023; 10 (1) Research ArticleOpen Access

Kappa Free Light Chain Biomarkers Are Efficient for the Diagnosis of Multiple Sclerosis

A Large Multicenter Cohort Study

Michael Levraut, Sabine Laurent-Chabalier, Xavier Ayrignac, Kévin Bigaut, Manon Rival, Sanae Squalli, Hélène Zéphir, Tifanie Alberto, Jean-David Pekar, Jonathan Ciron, Damien Biotti, Bénédicte Puissant-Lubrano, Jean-Philippe Camdessanché, Yannick Tholance, Olivier Casez, Bertrand Toussaint, Jeanne Marion, Thibault Moreau, Daniela Lakomy, Audrey Thomasset, Elisabeth Maillart, Delphine Sterlin, Aude Maurousset, Auriane Rocher, David Axel Laplaud, Edith Bigot-Corbel, Pierre-Olivier Bertho, Jean Pelletier, Joseph Boucraut, Pierre Labauge, Thierry Vincent, Jérôme De Sèze, Isabelle Jahn, Barbara Seitz-Polski, Eric Thouvenot, Christine Lebrun-Frenay; on behalf of Société Francophone de la Sclérose En Plaques (SFSEP)
First published November 14, 2022, DOI: https://doi.org/10.1212/NXI.0000000000200049
Michael Levraut
From the Unité de Recherche Clinique Cote d'Azur-UR2CA URRIS (M.L., C.L.-F.), Centre Hospitalier Universitaire de Nice; Département de Biostatistiques (S.L.-C.), Epidémiologie clinique et Santé Publique, Centre Hospitalier Universitaire de Nîmes; Département de Neurologie (X.A., P.L.), Centre Hospitalier Universitaire de Montpellier, Montpellier; Département de Neurologie (K.B., J.D.S.), Centre Hospitalier Universitaire de Strasbourg; Service de Neurologie (M.R., E.T.), Centre Hospitalier Universitaire de Nîmes; Laboratoire de Biochimie (S.S.), Centre Hospitalier Universitaire de Nîmes; Département de Neurologie (H.Z., T.A.), Centre Hospitalier Universitaire de Lille; Centre Hospitalier Universitaire de Lille (J.-D.P.), Service de Biochimie Automatisée, Protéines (UF 8833), Lille; Département de Neurologie (J.C., D.B.), Centre Hospitalier Universitaire de Toulouse, Hôpital Pierre-Paul Riquet, CRC-SEP, F-31059, Toulouse Cedex 9; Laboratoire d’Immunologie (B.P.-L.), Centre Hospitalier Universitaire de Toulouse; Département de Neurologie (J.-P.C.), Centre Hospitalier Universitaire de Saint-Etienne; Laboratoire de Biochimie (Y.T.), Centre Hospitalier Universitaire de Saint-Etienne; Département de Neurologie (O.C.), Centre Hospitalier Universitaire de Grenoble Alpes; Univ. Grenoble Alpes (B.T.), CNRS, UMR 5525, VetAgro Sup, Grenoble INP, CHU Grenoble Alpes, TIMC; Laboratoire de Biochimie (B.T., J.M.), Centre Hospitalier Universitaire de Grenoble Alpes; Département de Neurologie (T.M.), Centre Hospitalier de Dijon; Laboratoire de Biochimie (D.L., A.T.), Centre Hospitalier Universitaire de Dijon; Département de Neurologie (E.M.), Assistance-Publique des Hôpitaux de Paris, Centre Hospitalier Universitaire de la Pitié-Salpêtrière; Laboratoire d’Immunologie (D.S.), Assistance Publique des Hôpitaux de Paris, Centre Hospitalier Universitaire de la Pitié-Salpêtrière, Paris; Département de Neurologie (A.M., A.R.), Centre Hospitalier Universitaire de Tours; Université de Nantes (D.A.L.), C2RTI Inserm UMR1064, service de Neurologie, CHU Nantes; Département de Biochimie (E.B.-C., P.-O.B.), Centre Hospitalier Universitaire de Nantes; Aix Marseille Univ (J.P.), APHM, Hôpital de la Timone, Pôle de Neurosciences Cliniques, Service de Neurologie; Laboratoire de Biochimie (J.B.), Assistance Publique des Hôpitaux de Marseille, Centre Hospitalier Universitaire de La Conception; Laboratoire d’Immunologie (T.V.), Centre Hospitalier Universitaire de Montpellier; Laboratoire d’Immunologie (I.J.), Centre Hospitalier Universitaire Strasbourg; Laboratoire d’Immunologie (B.S.-P.), Centre Hospitalier Universitaire de Nice; Institut de Génomique Fonctionnelle (E.T.), Université de Montpellier, CNRS, INSERM, Montpellier; and Département de Neurologie (C.L.-F.), Centre Hospitalier Universitaire de Nice, France.
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Sabine Laurent-Chabalier
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Xavier Ayrignac
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Kévin Bigaut
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Manon Rival
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Sanae Squalli
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Hélène Zéphir
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Tifanie Alberto
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Jean-David Pekar
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Jonathan Ciron
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Damien Biotti
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Bénédicte Puissant-Lubrano
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Jean-Philippe Camdessanché
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Yannick Tholance
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Olivier Casez
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Bertrand Toussaint
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Jeanne Marion
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Thibault Moreau
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Daniela Lakomy
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Audrey Thomasset
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Elisabeth Maillart
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Delphine Sterlin
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Aude Maurousset
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Auriane Rocher
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David Axel Laplaud
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Edith Bigot-Corbel
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Pierre-Olivier Bertho
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Jean Pelletier
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Joseph Boucraut
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Pierre Labauge
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Thierry Vincent
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Jérôme De Sèze
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Isabelle Jahn
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Barbara Seitz-Polski
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Eric Thouvenot
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Christine Lebrun-Frenay
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Full PDF
Citation
Kappa Free Light Chain Biomarkers Are Efficient for the Diagnosis of Multiple Sclerosis
A Large Multicenter Cohort Study
Michael Levraut, Sabine Laurent-Chabalier, Xavier Ayrignac, Kévin Bigaut, Manon Rival, Sanae Squalli, Hélène Zéphir, Tifanie Alberto, Jean-David Pekar, Jonathan Ciron, Damien Biotti, Bénédicte Puissant-Lubrano, Jean-Philippe Camdessanché, Yannick Tholance, Olivier Casez, Bertrand Toussaint, Jeanne Marion, Thibault Moreau, Daniela Lakomy, Audrey Thomasset, Elisabeth Maillart, Delphine Sterlin, Aude Maurousset, Auriane Rocher, David Axel Laplaud, Edith Bigot-Corbel, Pierre-Olivier Bertho, Jean Pelletier, Joseph Boucraut, Pierre Labauge, Thierry Vincent, Jérôme De Sèze, Isabelle Jahn, Barbara Seitz-Polski, Eric Thouvenot, Christine Lebrun-Frenay
Neurol Neuroimmunol Neuroinflamm Jan 2023, 10 (1) e200049; DOI: 10.1212/NXI.0000000000200049

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    Figure 1 Comparison of the Different FLC Biomarkers Values Between Groups

    CIS = clinically isolated syndrome; CSF KFLC = CSF kappa free light chains; CSF LFLC = CSF lambda free light chains; IF = intrathecal fraction; KFLC IF = kappa free light chains IF; KFLC index = kappa free light chains index; LFLC IF = lambda free light chains intrathecal fraction; LFLC index = lambda free light chains index; MS = multiple sclerosis; NINDC = noninflammatory neurologic disorder controls; ns = nonsignificant; OIND = other inflammatory neurologic disorder. *p < 0.05, **p < 0.01, ***p < 0.001.

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    Figure 2 FLC Biomarkers ROC Curve Analysis and Ability to Separate MS (±CIS) From Other Control Populations

    Panel A shows diagnostic performances to separate MS and CIS from patients with NINDC and OIND (n = 1,621 for KFLC biomarkers and n = 811 for LFLC biomarkers). Panel B shows diagnostic performances to separate MS from patients with SC (n = 842 patients for KFLC biomarkers and n = 415 for LFLC biomarkers). Panel C shows diagnostic performances to separate MS from patients with NIND (n = 1,067 for KFLC biomarkers and n = 541 for LFLC biomarkers). Panel D shows diagnostic performances to separate MS from patients with OIND (n = 972 patients for KFLC biomarkers and n = 479 for LFLC biomarkers). CIS = clinically isolated syndrome; FLC = free light chain; IF = intrathecal fraction; KFLC = Kappa free light chain; LFLC = lambda free light chain; NINDS = noninflammatory CNS disorder control; OIND = other inflammatory CNS disorder.

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