Skip to main content
Advertisement
  • Neurology.org
  • Journals
    • Neurology
    • Clinical Practice
    • Education
    • Genetics
    • Neuroimmunology & Neuroinflammation
  • Online Sections
    • Neurology Video Journal Club
    • Neurology: Neuroimmunology & Neuroinflammation COVID-19 Article Hub
    • Inclusion, Diversity, Equity, Anti-racism, & Social Justice (IDEAS)
    • Innovations in Care Delivery
    • Practice Buzz
    • Practice Current
    • Residents & Fellows
    • Without Borders
  • Collections
    • COVID-19
    • Disputes & Debates
    • Health Disparities
    • Infographics
    • Null Hypothesis
    • Patient Pages
    • Topics A-Z
    • Translations
  • Podcast
  • CME
  • About
    • About the Journals
    • Contact Us
    • Editorial Board
  • Authors
    • Submit a Manuscript
    • Author Center

Advanced Search

Main menu

  • Neurology.org
  • Journals
    • Neurology
    • Clinical Practice
    • Education
    • Genetics
    • Neuroimmunology & Neuroinflammation
  • Online Sections
    • Neurology Video Journal Club
    • Neurology: Neuroimmunology & Neuroinflammation COVID-19 Article Hub
    • Inclusion, Diversity, Equity, Anti-racism, & Social Justice (IDEAS)
    • Innovations in Care Delivery
    • Practice Buzz
    • Practice Current
    • Residents & Fellows
    • Without Borders
  • Collections
    • COVID-19
    • Disputes & Debates
    • Health Disparities
    • Infographics
    • Null Hypothesis
    • Patient Pages
    • Topics A-Z
    • Translations
  • Podcast
  • CME
  • About
    • About the Journals
    • Contact Us
    • Editorial Board
  • Authors
    • Submit a Manuscript
    • Author Center
  • Home
  • Articles
  • Issues
  • COVID-19 Article Hub
  • Infographics & Video Summaries

User menu

  • My Alerts
  • Log in

Search

  • Advanced search
Neurology Neuroimmunology & Neuroinflammation
Home
A peer-reviewed clinical and translational neurology open access journal
  • My Alerts
  • Log in
Site Logo
  • Home
  • Articles
  • Issues
  • COVID-19 Article Hub
  • Infographics & Video Summaries

Share

March 2020; 7 (2) ArticleOpen Access

Frequency of myelin oligodendrocyte glycoprotein antibody in multiple sclerosis

A multicenter cross-sectional study

Álvaro Cobo-Calvo, Hyacintha d'Indy, Anne Ruiz, View ORCID ProfileNicolas Collongues, Laurent Kremer, Françoise Durand-Dubief, Fabien Rollot, Romain Casey, Sandra Vukusic, Jérôme De Seze, Romain Marignier
First published December 13, 2019, DOI: https://doi.org/10.1212/NXI.0000000000000649
Álvaro Cobo-Calvo
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hyacintha d'Indy
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anne Ruiz
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicolas Collongues
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Nicolas Collongues
Laurent Kremer
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Françoise Durand-Dubief
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fabien Rollot
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Romain Casey
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sandra Vukusic
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jérôme De Seze
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Romain Marignier
From the Service de Neurologie (Á.C.-C., F.D.-D., S.V., R.M.), Sclérose en Plaques, Pathologies de La Myéline et Neuro-inflammation, Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, France; Centre de Référence pour Les Maladies Inflammatoires Rares Du Cerveau et de La Moelle (MIRCEM) (Á.C.-C., F.D.-D., S.V., R.M.), Hôpital Neurologique Pierre Wertheimer Hospices Civils de Lyon, Lyon, France; Centre de Recherche en Neurosciences de Lyon (Á.C.-C., H.I., A.R., R.M.), U1028 INSERM, UMR5292 CNRS, Lyon, France; Département de Neurologie (N.C., L.K., J.D.S.), Centre Hospitalier Universitaire de Strasbourg, France; Biopathologie de La Myéline (N.C., J.D.S.), Neuroprotection et Stratégies Thérapeutiques, INSERM U1119, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg, France; Centre D'investigation Clinique (N.C., J.D.S.), INSERM U1434, Centre Hospitalier Universitaire de Strasbourg, France; Centre de Recherche en Neurosciences de Lyon (F.R., R.C., S.V.), Observatoire Français de La Sclérose en Plaques, INSERM 1028 et CNRS UMR5292, Lyon, France; and Université Claude Bernard Lyon 1 (F.R., R.C., S.V.), F-69000 Lyon, France; Hospices Civils de Lyon, Lyon, France.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Full PDF
Citation
Frequency of myelin oligodendrocyte glycoprotein antibody in multiple sclerosis
A multicenter cross-sectional study
Álvaro Cobo-Calvo, Hyacintha d'Indy, Anne Ruiz, Nicolas Collongues, Laurent Kremer, Françoise Durand-Dubief, Fabien Rollot, Romain Casey, Sandra Vukusic, Jérôme De Seze, Romain Marignier
Neurol Neuroimmunol Neuroinflamm Mar 2020, 7 (2) e649; DOI: 10.1212/NXI.0000000000000649

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Permissions

Make Comment

See Comments

Downloads
1281

Share

  • Article
  • Figures & Data
  • Info & Disclosures
Loading

Abstract

Objective To address the frequency of myelin oligodendrocyte glycoprotein (MOG) antibody (Ab) in an unselected large cohort of adults with MS.

Methods This is a cross-sectional study in 2 MS expert centers (Lyon and Strasbourg University Hospitals, France) between December 1, 2017, and June 31, 2018. Patients aged ≥18 years with a definite diagnosis of MS according to 2010 McDonald criteria were tested for MOG-Ab by using a cell-based assay (CBA) in Lyon and subsequently included. Positive samples were tested by investigators blinded to the first result with a second assay in a different laboratory (Barcelona, Spain) by using the same plasmid and secondary Ab.

Results Serum samples from 685 consecutive patients with MS were analyzed for MOG-Ab. Median disease duration at sampling was 11.5 (interquartile range, 5.8–17.7) years, and 72% were women. Two (0.3%) patients resulted to be MOG-Ab-positive. The 2 patients were women aged 42 and 38 at disease onset and were diagnosed with secondary and primary progressive forms of MS, respectively. This positive result was confirmed by the CBA in Barcelona.

Conclusion Our findings indicate that MOG-Ab are exceptional in MS phenotype, suggesting that the MOG-Ab testing should not be performed in typical MS presentation.

Glossary

Ab=
antibody;
CBA=
cell-based assay;
DMT=
disease-modifying treatment;
EDMUS=
Eugene Devic Foundation against Multiple Sclerosis;
IQR=
interquartile range;
MOG=
myelin oligodendrocyte glycoprotein;
NMO=
neuromyelitis optica;
OFSEP=
Observatoire Français de la Sclérose En Plaques;
ON=
optic neuritis

In adults, myelin oligodendrocyte glycoprotein (MOG) antibodies (Ab) are mainly found in patients with a neuromyelitis optica clinical phenotype, i.e., optic neuritis (ON) or myelitis isolated or in combination.1

A recent review pooling patients from all available MOG-Ab studies found that 24 of 1,608 (1.5%) and 105 of 1771 (6%) patients with a confirmed diagnosis of MS had MOG-Ab by using cell-based assays (CBAs) with immunofluorescence or fluorescence-activated cell sorting (FACS), respectively.2 However, the sample size of patients with MS included as controls in these studies is limited, patients were usually preselected, and most importantly, such studies have not been designed to ascertain the specific value of MOG-Ab in patients with a definite diagnosis of MS.3,–,6 Thus, to draw definitive conclusions about antibody, specificity should be prevented. The only study aimed at determining the frequency of MOG-Ab in MS included 200 selected patients with MS, all primary or secondary progressive forms, and all tested negative.7 Therefore, whether MOG-Ab can be present in MS and in what proportion has never been precisely evaluated.

In the present study, we addressed the frequency of MOG-Ab in a large sample of unselected patients with MS using a highly specific assay.

Methods

Study design

We performed a cross-sectional study in 2 MS expert centers (Lyon and Strasbourg University Hospitals, France) between December 1, 2017, and June 31, 2018. All patients aged ≥18 years with a definite diagnosis of MS according to 2010 McDonald criteria. Patients included were visited consecutively as part of their routine clinical practice in the day care unit.8

Clinical information was provided in specific case report forms by a neurologist with expertise in neuroinflammatory disorders and entered in the Eugene Devic Foundation against Multiple Sclerosis (EDMUS) database.9 Demographic data (sex and Caucasian ethnicity) and age at the onset of disease and disease duration at sampling were collected. MS disease subtype (clinically isolated syndrome, relapsing-remitting, secondary or primary progressive MS) was also reported. Relapses within the month before sampling, as well as corticosteroids and disease-modifying treatments (DMTs) at the time of sampling, were collected. Patients on anti-CD20 were considered on-treatment in the 6 months after the last infusion. Medical charts of MOG-Ab-positive cases were reviewed in detail by expert clinicians (A.C.-C., R.M., and J.D.S.).

Live CBAs

HEK293 cells were transfected with pEGFP-N1-hMOG plasmid. Serum samples were used at a dilution of 1:640. Allophycocyanin-Goat IgG-Fcγ fragment-specific was used as a secondary antibody and signal intensity evaluation was performed with FACS. As recommended,10 positive samples were tested by investigators blinded to the first result with a second assay in Barcelona by using the same plasmid and secondary antibody4 (supplementary data, links.lww.com/NXI/A169).

Standard protocol approvals, registrations, and patient consents

All participants included in the present study belong to the national French registry designated as Observatoire Français de la Sclérose En Plaques9 and signed informed consent to have their medical data collected in routine practice used after anonymization and aggregation for research purposes. MOG-Ab were performed as part of the clinical routine evaluation; thus, no other specific consent was required.

Data availability

Anonymized data can be made available on reasonable request to the corresponding author.

Results

Serum samples from 685 patients with MS were analyzed for MOG-Ab during the period of this study. The median age at disease onset was 28.4 (interquartile range [IQR], 22.1–37.2) years, and the median disease duration at sampling was 11.5 (IQR, 5.8–17.7) years. Seventy-two per cent were women, and 80.6% Caucasians (table 1). Fifty (7.3%) patients had relapsed within the month before sampling. Forty-six (6.7%) and 440 (64.2%) had received corticosteroids and DMTs within the month previous to sampling, respectively. Additional characterization of the MS cohort is depicted in table 1 and table e-1, links.lww.com/NXI/A171.

View this table:
  • View inline
  • View popup
  • Download powerpoint
Table 1

Epidemiologic and clinical features of the MS cohort

Two (0.3%) female patients, aged 42 and 38 at MS onset, were found MOG-Ab-positive after 26 and 11 years of disease duration, respectively. One patient was diagnosed with a secondary progressive MS and the other with a primary progressive MS, with no history of ON or myelitis. Clinical information of MOG-Ab-positive patients is shown in table 2 and figure e-1, links.lww.com/NXI/A170.

View this table:
  • View inline
  • View popup
  • Download powerpoint
Table 2

Clinical features of patients with MS with positive MOG-Ab

Discussion

n the present cohort of unselected definite patients with MS, only 2 (0.3%) were found MOG-Ab-positive, among 685 patients tested.

Differential diagnosis at the onset of disease in patients with MOG-Ab-positive and in those with MS is challenging because a proportion may present with overlapping features, i.e., ON involvement, short myelitis, or MS-like brain lesions. Such an overlap raises the question of whether patients with MS should be tested widely for MOG-Ab. The use of accurate antibody assays is highly recommended to discriminate true positive cases. Currently, live CBA using human full-length MOG and restricting with IgG1 or IgG-Fcγ fragment-specific as a secondary antibody is the most accurate detection method.10 By using these techniques, we achieved full agreement between Lyon and Barcelona laboratories. Both centers used similar approaches except for the antibody lecture: FACS in Lyon and immunocytochemistry in Barcelona.

The present study was conducted by 2 French referral centers for neuroinflammatory disorders that follow internationally well-validated criteria for MS diagnosis and have a recognized expertise in other less frequent CNS demyelinating diseases. Therefore, if we consider this high clinical sensibility to discriminate rare diseases from typical MS and the fulfillment of 2010 McDonald criteria in all the patients included, we could assume that the 2 MOG-Ab-positive patients yielded a false positive result. Indeed, both patients had no typical symptoms of MOG-Ab-associated disease1 but a genuine progressive phenotype with typical MS features. Our results are in line with those recently obtained by the Oxford and Mayo group displaying 100% and 99.6% of specificity, respectively, although a preselected MS cohort was included in this study.6 The only study evaluating the frequency of MOG-Ab in progressive MS did not find any positive result by using a similar method than us.7 However, this is the first study focused on different MS subtypes whose major strength lies on the large sample size allowing for a well-powered investigation. Therefore, the absence of a positive result in 99.7% of patients with typical MS together with the agreement of the results between centers in the MOG-Ab-positive cases supports the high specificity of the antibody testing methodology.

Certain limitations must be addressed. MOG-Ab titers may vary depending on the phase of the disease being higher during relapse and lower in the remission phase.1 This fluctuation over time may underestimate the real frequency of MOG-Ab and increase the risk of false negative result when performing cross-sectional studies. Moreover, corticosteroids or long-term treatment might also have an influence over titers.

In conclusion, the present study revealed low (0.3%) frequency for MOG-Ab positivity in patients with MS by using highly specific assays. Our results have important clinical implications for neurologists in daily clinical practice, and we do not recommend MOG-Ab testing in patients with MS fulfilling 2010 McDonald criteria, with typical features.

Study funding

This work was conducted using data from the Observatoire Français de la Sclérose en Plaques (OFSEP), which is supported by a grant provided by the French State and handled by the “Agence Nationale de la Recherche,” within the framework of the “Investments for the Future” program, under the reference ANR-10-COHO-002 Observatoire Français de la Sclérose en Plaques (OFSEP), the EDMUS Foundation, and Déchaine Ton Coeur.

Disclosure

F. Durand-Dubief serves on a scientific advisory board for Merck Serono and has received funding for travel and honoraria from Biogen, Idec, Merck Serono, Novartis, Sanofi Genzyme, Roche, and Teva Pharm. S. Vukusic has received consulting and lecturing fees, travel grants, and research support from Biogen, GeNeuro, Genzyme, Novartis, Merck Serono, Roche, Sanofi-Aventis and Teva Pharm. R. Marignier has received consulting and lecturing fees, travel grants, and research support from Bayer-Schering, Biogen, Idec, Genzyme, Novartis, Merck Serono, Roche, Sanofi-Aventis, and Teva Pharma. Go to Neurology.org/NN for full disclosures

Acknowledgment

The authors thank Dr. Albert Saiz (Center of Neuroimmunology, Hospital Clinic, Barcelona, Spain) for having performed different antibody testing. The authors thank the group of NeuroBioTec from Hôpital Civils de Lyon for supporting this study.

Appendix Authors

Table
Table

Footnotes

  • Go to Neurology.org/NN for full disclosures. Funding information is provided at the end of the article

  • The Article Processing Charge was funded by the authors.

  • Received September 30, 2019.
  • Accepted in final form November 5, 2019.
  • Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND), which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

References

  1. 1.↵
    1. Cobo-Calvo A,
    2. Ruiz A,
    3. Maillart E, et al
    . Clinical spectrum and prognostic value of CNS MOG autoimmunity in adults: the MOGADOR study. Neurology 2018;90:e1858–e1869.
    OpenUrl
  2. 2.↵
    1. Reindl M,
    2. Waters P
    . Myelin oligodendrocyte glycoprotein antibodies in neurological disease. Nat Rev Neurol 2019;15:89–102.
    OpenUrl
  3. 3.↵
    1. Jarius S,
    2. Ruprecht K,
    3. Kleiter I, et al
    . MOG-IgG in NMO and related disorders: a multicenter study of 50 patients. Part 1: frequency, syndrome specificity, influence of disease activity, long-term course, association with AQP4-IgG, and origin. J Neuroinflammation 2016;13:279.
    OpenUrlCrossRefPubMed
  4. 4.↵
    1. Höftberger R,
    2. Sepulveda M,
    3. Armangue T, et al
    . Antibodies to MOG and AQP4 in adults with neuromyelitis optica and suspected limited forms of the disease. Mult Scler 2015;21:866–874.
    OpenUrlCrossRefPubMed
  5. 5.↵
    1. Hacohen Y,
    2. Absoud M,
    3. Deiva K, et al
    . Myelin oligodendrocyte glycoprotein antibodies are associated with a non-MS course in children. Neurol Neuroimmunol Neuroinflamm 2015;2:e81. doi: 10.1212/NXI.0000000000000081.
    OpenUrlAbstract/FREE Full Text
  6. 6.↵
    1. Waters PJ,
    2. Komorowski L,
    3. Woodhall M, et al
    . A multicenter comparison of MOG-IgG cell-based assays. Neurology 2019;92:e1250–e1255.
    OpenUrl
  7. 7.↵
    1. Jarius S,
    2. Ruprecht K,
    3. Stellmann JP, et al
    . MOG-IgG in primary and secondary chronic progressive multiple sclerosis: a multicenter study of 200 patients and review of the literature. J Neuroinflammation 2018;15:88.
    OpenUrl
  8. 8.↵
    1. Polman CH,
    2. Reingold SC,
    3. Banwell B, et al
    . Diagnostic criteria for multiple sclerosis: 2010 revisions to the McDonald criteria. Ann Neurol 2011;69:292–302.
    OpenUrlCrossRefPubMed
  9. 9.↵
    1. Vukusic S,
    2. Casey R,
    3. Rollot F, et al
    . Observatoire Français de la Sclérose en Plaques (OFSEP): A unique multimodal nationwide MS registry in France. Mult Scler Epub 2018 Dec 13.
  10. 10.↵
    1. Jarius S,
    2. Paul F,
    3. Aktas O, et al
    . MOG encephalomyelitis: international recommendations on diagnosis and antibody testing. J Neuroinflammation 2018;15:134.
    OpenUrl

Letters: Rapid online correspondence

No comments have been published for this article.
Comment

REQUIREMENTS

If you are uploading a letter concerning an article:
You must have updated your disclosures within six months: http://submit.neurology.org

Your co-authors must send a completed Publishing Agreement Form to Neurology Staff (not necessary for the lead/corresponding author as the form below will suffice) before you upload your comment.

If you are responding to a comment that was written about an article you originally authored:
You (and co-authors) do not need to fill out forms or check disclosures as author forms are still valid
and apply to letter.

Submission specifications:

  • Submissions must be < 200 words with < 5 references. Reference 1 must be the article on which you are commenting.
  • Submissions should not have more than 5 authors. (Exception: original author replies can include all original authors of the article)
  • Submit only on articles published within 6 months of issue date.
  • Do not be redundant. Read any comments already posted on the article prior to submission.
  • Submitted comments are subject to editing and editor review prior to posting.

More guidelines and information on Disputes & Debates

Compose Comment

More information about text formats

Plain text

  • No HTML tags allowed.
  • Web page addresses and e-mail addresses turn into links automatically.
  • Lines and paragraphs break automatically.
Author Information
NOTE: The first author must also be the corresponding author of the comment.
First or given name, e.g. 'Peter'.
Your last, or family, name, e.g. 'MacMoody'.
Your email address, e.g. higgs-boson@gmail.com
Your role and/or occupation, e.g. 'Orthopedic Surgeon'.
Your organization or institution (if applicable), e.g. 'Royal Free Hospital'.
Publishing Agreement
NOTE: All authors, besides the first/corresponding author, must complete a separate Publishing Agreement Form and provide via email to the editorial office before comments can be posted.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

Vertical Tabs

You May Also be Interested in

Back to top
  • Article
    • Abstract
    • Glossary
    • Methods
    • Results
    • Discussion
    • Study funding
    • Disclosure
    • Acknowledgment
    • Appendix Authors
    • Footnotes
    • References
  • Figures & Data
  • Info & Disclosures
Advertisement

Preferences and User Experiences of Wearable Devices in Epilepsy A Systematic Review and Mixed-Methods Synthesis

Dr. Daniel Friedman and Dr. Sharon Chiang

► Watch

Related Articles

  • No related articles found.

Topics Discussed

  • Multiple sclerosis
  • Devic's syndrome

Alert Me

  • Alert me when eletters are published
Neurology - Neuroimmunology Neuroinflammation: 10 (2)

Articles

  • Articles
  • Issues
  • Popular Articles

About

  • About the Journals
  • Ethics Policies
  • Editors & Editorial Board
  • Contact Us
  • Advertise

Submit

  • Author Center
  • Submit a Manuscript
  • Information for Reviewers
  • AAN Guidelines
  • Permissions

Subscribers

  • Subscribe
  • Sign up for eAlerts
  • RSS Feed
Site Logo
  • Visit neurology Template on Facebook
  • Follow neurology Template on Twitter
  • Visit Neurology on YouTube
  • Neurology
  • Neurology: Clinical Practice
  • Neurology: Education
  • Neurology: Genetics
  • Neurology: Neuroimmunology & Neuroinflammation
  • AAN.com
  • AANnews
  • Continuum
  • Brain & Life
  • Neurology Today

Wolters Kluwer Logo

Neurology: Neuroimmunology & Neuroinflammation
Online ISSN: 2332-7812

© 2023 American Academy of Neurology

  • Privacy Policy
  • Feedback
  • Advertise