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September 2020; 7 (5) ArticleOpen Access

Painful trigeminal neuropathy associated with anti-Plexin D1 antibody

Takayuki Fujii, Ryo Yamasaki, Yukino Miyachi, Kyoko Iinuma, Yu Hashimoto, Noriko Isobe, Takuya Matsushita, Jun-ichi Kira
First published June 25, 2020, DOI: https://doi.org/10.1212/NXI.0000000000000819
Takayuki Fujii
From the Department of Neurological Therapeutics (T.F., N.I.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Department of Neurology (R.Y., Y.M., K.I., Y.H., T.M., J.K.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Translational Neuroscience Center (J.K.), Graduate School of Medicine, and School of Pharmacy at Fukuoka, International University of Health and Welfare, Ookawa, Fukuoka; and Department of Neurology (J.K.), Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Chuou-ku, Japan.
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Ryo Yamasaki
From the Department of Neurological Therapeutics (T.F., N.I.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Department of Neurology (R.Y., Y.M., K.I., Y.H., T.M., J.K.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Translational Neuroscience Center (J.K.), Graduate School of Medicine, and School of Pharmacy at Fukuoka, International University of Health and Welfare, Ookawa, Fukuoka; and Department of Neurology (J.K.), Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Chuou-ku, Japan.
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Yukino Miyachi
From the Department of Neurological Therapeutics (T.F., N.I.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Department of Neurology (R.Y., Y.M., K.I., Y.H., T.M., J.K.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Translational Neuroscience Center (J.K.), Graduate School of Medicine, and School of Pharmacy at Fukuoka, International University of Health and Welfare, Ookawa, Fukuoka; and Department of Neurology (J.K.), Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Chuou-ku, Japan.
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Kyoko Iinuma
From the Department of Neurological Therapeutics (T.F., N.I.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Department of Neurology (R.Y., Y.M., K.I., Y.H., T.M., J.K.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Translational Neuroscience Center (J.K.), Graduate School of Medicine, and School of Pharmacy at Fukuoka, International University of Health and Welfare, Ookawa, Fukuoka; and Department of Neurology (J.K.), Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Chuou-ku, Japan.
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Yu Hashimoto
From the Department of Neurological Therapeutics (T.F., N.I.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Department of Neurology (R.Y., Y.M., K.I., Y.H., T.M., J.K.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Translational Neuroscience Center (J.K.), Graduate School of Medicine, and School of Pharmacy at Fukuoka, International University of Health and Welfare, Ookawa, Fukuoka; and Department of Neurology (J.K.), Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Chuou-ku, Japan.
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Noriko Isobe
From the Department of Neurological Therapeutics (T.F., N.I.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Department of Neurology (R.Y., Y.M., K.I., Y.H., T.M., J.K.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Translational Neuroscience Center (J.K.), Graduate School of Medicine, and School of Pharmacy at Fukuoka, International University of Health and Welfare, Ookawa, Fukuoka; and Department of Neurology (J.K.), Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Chuou-ku, Japan.
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Takuya Matsushita
From the Department of Neurological Therapeutics (T.F., N.I.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Department of Neurology (R.Y., Y.M., K.I., Y.H., T.M., J.K.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Translational Neuroscience Center (J.K.), Graduate School of Medicine, and School of Pharmacy at Fukuoka, International University of Health and Welfare, Ookawa, Fukuoka; and Department of Neurology (J.K.), Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Chuou-ku, Japan.
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Jun-ichi Kira
From the Department of Neurological Therapeutics (T.F., N.I.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Department of Neurology (R.Y., Y.M., K.I., Y.H., T.M., J.K.), Neurological Institute, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka; Translational Neuroscience Center (J.K.), Graduate School of Medicine, and School of Pharmacy at Fukuoka, International University of Health and Welfare, Ookawa, Fukuoka; and Department of Neurology (J.K.), Brain and Nerve Center, Fukuoka Central Hospital, International University of Health and Welfare, Chuou-ku, Japan.
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Citation
Painful trigeminal neuropathy associated with anti-Plexin D1 antibody
Takayuki Fujii, Ryo Yamasaki, Yukino Miyachi, Kyoko Iinuma, Yu Hashimoto, Noriko Isobe, Takuya Matsushita, Jun-ichi Kira
Neurol Neuroimmunol Neuroinflamm Sep 2020, 7 (5) e819; DOI: 10.1212/NXI.0000000000000819

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Abstract

Objective To determine whether anti-Plexin D1 antibody (Plexin D1-immunoglobulin G [IgG]), which is associated with limb and trunk neuropathic pain (NP) and binds to pain-conducting small unmyelinated dorsal root ganglion (DRG) neurons, exists in patients with idiopathic painful trigeminal neuropathy (IPTN) and whether Plexin D1-IgG binds to trigeminal ganglion (TG) neurons.

Methods We enrolled 21 consecutive patients with IPTN and 35 age- and sex-matched controls without NP (25 healthy persons and 10 with neurodegenerative diseases). We measured serum Plexin D1-IgG using a mouse DRG tissue–based indirect immunofluorescence assay (IFA) and by Western blotting (WB) using a recombinant human Plexin D1 (rhPlexin D1) accompanied by immunoadsorption tests with rhPlexin D1. The reactivity of Plexin D1-IgG toward mouse TG, brain, heart, and kidney was assessed by tissue-based IFAs.

Results Serum Plexin D1-IgG was detected more frequently in IPTN than in controls by both IFA and WB (14.3% vs 0%, p = 0.048). Three Plexin D1-IgG–positive patients also had limb or trunk NP and commonly showed tongue pain. In tissue-based IFAs, IgG from 2 Plexin D1-IgG–positive patients immunostained small TG neurons, which was prevented by preincubation with rhPlexin D1. Moreover, Plexin D1-IgG immunostaining mostly colocalized with isolectin B4-positive pain-conducting unmyelinated TG neurons. IFAs of other tissues with the same IgG revealed weak immunoreactivity only in endothelial cells, which was prevented by preincubation with rhPlexin D1.

Conclusions Plexin D1-IgG, which binds to pain-conducting small TG neurons in addition to DRG neurons, can be present in IPTN as well as limb and trunk NP.

Glossary

DRG=
dorsal root ganglion;
HC=
healthy control;
IB4=
isolectin B4;
ICHD-3=
International Classification of Headache Disorders 3rd edition;
IFA=
immunofluorescence assay;
IgG=
immunoglobulin G;
IPTN=
idiopathic painful trigeminal neuropathy;
IVIg=
IV immunoglobulin;
NCS=
nerve conduction study;
NFH=
neurofilament heavy chain;
NP=
neuropathic pain;
PE=
plasma exchange;
rhPlexin D1=
recombinant human Plexin D1;
TG=
trigeminal ganglion;
TN=
trigeminal nerve;
WB=
Western blotting

Painful trigeminal neuropathy (PTN) presents with facial pain that coincides with the distribution of the trigeminal nerves (TNs). PTN develops in a variety of underlying conditions, but its pathomechanism is frequently undetermined. The International Classification of Headache Disorders 3rd edition (ICHD-3) defines such cases with unknown mechanism as idiopathic PTN (IPTN).1

We recently reported anti-Plexin D1 antibody (Plexin D1-immunoglobulin G [IgG]) in the sera of 10% of patients with limb and trunk neuropathic pain (NP).2 Plexin D1-IgG binds to and exerts cytotoxic effects against isolectin B4 (IB4)-positive pain-conducting small unmyelinated dorsal root ganglion (DRG) neurons.2 NP was improved in all Plexin D1-IgG–positive cases treated with plasma exchange.2 NP occasionally manifests facial pain; therefore, we assessed whether Plexin D1-IgG exists in patients with IPTN and determined whether Plexin D1-IgG binds to trigeminal ganglion (TG) neurons.

Methods

Patients

We enrolled 21 consecutive patients with IPTN who attended our clinic between 2008 and 2019, and we reviewed their medical records. An IPTN diagnosis was based on the established criteria1: unilateral or bilateral facial pain colocalizing with one or both TNs, clinically evident positive (hyperalgesia and allodynia) and/or negative (hypesthesia and hypoalgesia) signs of TN dysfunction, no identified cause, and not better accounted for by another ICHD-3 diagnosis. Patients with some underlying diseases were not excluded unless the mechanism causing PTN was established. As controls, 35 age- and sex-matched subjects without NP were used (25 healthy persons and 10 with neurodegenerative diseases).

Standard protocol approvals, registrations, and patient consents

This study was approved by the Ethical Committee of Kyushu University (#29-40 and #30-164). All patients and controls provided written informed consent. Animal experiments were performed according to the protocols approved by the Institutional Animal Care and Use Committee at Kyushu University (A19-109).

Plexin D1-IgG detection

For all participants, serum Plexin D1-IgG was measured by both mouse DRG tissue–based indirect immunofluorescence assays (IFAs) and Western blotting (WB) using recombinant human Plexin D1 (rhPlexin D1) accompanied by immunoadsorption tests with rhPlexin D1 (R&D Systems, Minneapolis).2 Before testing, patients' sera were preabsorbed with mouse liver powder (Rockland, Gilbertsville).3

Mouse tissue–based IFAs

IFAs were conducted using patient IgG and 4-µm paraffin sections processed from 10% buffered formalin-fixed adult male C57BL/6 mouse tissues (10–12 weeks old).2 We also performed double immunostaining of TG neurons with patient IgG and Alexa Fluor 594–conjugated anti-IB4 (Thermo Fisher Scientific, Waltham, 1:1,000) and with patient IgG and anti-neurofilament heavy chain (NFH) (Covance, Princeton, 1:500).

Data availability

Any data not published within the article will be shared in anonymized form by request from any qualified investigator.

Results

Detection of Plexin D1-IgG in IPTN

Serum Plexin D1-IgG detected by both IFA and WB was more frequent in patients with IPTN than in controls (3/21 [14.3%] vs 0/35 [0%], p = 0.048) (figure 1 and table). The overall coincidence rate of positive WB and IFA results was 98.2% (55/56, 1 control had an immunoreactive IgG band on WB but negative immunoreactivity to mouse DRG). Three patients who were Plexin D1-IgG–positive also had limb or trunk NP and commonly showed tongue pain, which was more frequent compared with patients with IPTN who were Plexin D1-IgG–negative (100% vs 11.1%, p = 0.03) (table). Otherwise, no difference was found in any parameter examined between the 2 groups. Here, we present 3 IPTN cases with Plexin D1-IgG.

Figure 1
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Figure 1 Detection of Plexin D1-IgG by IFA using mouse DRG and WB with rhPlexin D1

(A) IFA with mouse DRG for case 1. IgG from case 1 (green) bound to small DRG neurons (upper images). The immunostaining (green) of small DRG neurons by IgG from case 1 was prevented by preincubation with rhPlexin D1 (2 µg/mL) (lower images). Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (B) IFA with mouse DRG for case 2. IgG from case 2 (green) bound to small DRG neurons (upper images). The immunostaining (green) of small DRG neurons by IgG from case 2 was prevented by preincubation with rhPlexin D1 (2 µg/mL) (lower images). Nuclei are counterstained with DAPI (blue). (C) WB analysis using rhPlexin D1 and IgG from cases 1, 2, and 3, 3 patients with IPTN without Plexin D1-IgG, 3 healthy controls, 3 patients with neurodegenerative diseases, and a commercial anti-human Plexin D1 antibody. WB of rhPlexin D1 with IgG samples from cases 1, 2, and 3 showed a common immunoreactive band between 150 and 250 kDa, similar to the commercial anti-human Plexin D1 antibody (R&D Systems, Minneapolis). All other samples were nonreactive. Scale bars: (A and B) = 50 µm. DRG = dorsal root ganglion; IFA = immunofluorescence assay; IPTN = idiopathic painful trigeminal neuropathy; ND = neurodegenerative diseases; Plexin D1-IgG = anti-Plexin D1 antibody; rhPlexin D1 = recombinant human Plexin D1; WB = Western blotting.

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Table

Clinical features of patients with IPTN with and without Plexin D1-IgG

Case 1

A 64-year-old woman had a 15-year history of persistent pain and numbness in her tongue and perioral area, which gradually spread to all 3 divisions of bilateral TNs and both upper limbs. Neurologic examination revealed a decreased sensation to pinprick and light touch in all TN divisions and the upper limbs. A nerve conduction study (NCS) showed decreased sensory action potentials in her upper limbs. Bilateral blink reflexes showed delayed R1 and R2 responses. IV immunoglobulin (IVIg) administration did not improve her symptoms.

Case 2

Two months before admission, a 46-year-old woman with atopic myelitis developed persistent pain and numbness of the tongue, face, and a girdle-like sensation around mid-trunk. Neurologically, she had hypesthesia in the tongue, face, and mid-trunk on admission. Spinal cord MRI revealed T2 hyperintense lesions at the level of Th8, whereas cranial MRI showed no relevant lesions. Her pain in the tongue, face, and mid-trunk was relieved by a combined administration of corticosteroids, azathioprine, and analgesics.

Case 3

An 81-year-old woman with a history of hepatitis B virus–related liver cancer presented with tingling in her tongue and the soles of her feet, which she had experienced over the preceding 12 years. Neurologic examination revealed a decreased sensation to pinprick in her tongue and feet soles but preserved vibratory sensation and normal deep tendon reflexes. NCS showed normal findings. Therefore, her diagnosis was small fiber neuropathy of unknown etiology. She received an analgesic agent only without immunotherapy to avoid hepatitis B virus reactivation.

Tissue-based IFA

IgG from cases 1 and 2 bound to small TG neurons in IFAs (figure 2, A and B). Preincubation with rhPlexin D1 prevented the staining of small TG neurons by the patients' IgG (figure 2, A and B), indicating that Plexin D1-IgG bound to small TG neurons. Dual labeling revealed that IB4 immunostaining, a marker for pain-conducting unmyelinated neurons, mostly colocalized with patient IgG binding (figure 2C). Conversely, patient IgG did not colocalize with NFH, a marker for myelinated neurons (figure 2D). These findings indicated that Plexin D1-IgG binds to pain-conducting unmyelinated TG neurons in addition to DRG.2 IFAs of mouse brain, heart, and kidney tissues with patient IgG demonstrated weak immunoreactivity only in endothelial cells, which was prevented by preincubation with rhPlexin D1 (figure 2E).

Figure 2
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Figure 2 IFA using mouse tissues and IgG from patients with IPTN with Plexin D1-IgG

(A) IFA with mouse TG for case 1. IgG from case 1 (green) bound to small TG neurons (left image). The immunostaining (green) of small TG neurons by IgG from case 1 was prevented by preincubation with rhPlexin D1 (2 µg/mL) (right image). Nuclei are counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). (B) IFA with mouse TG for case 2. IgG from case 2 (green) bound to small TG neurons (left image). The immunostaining (green) of small TG neurons by IgG from case 2 was prevented by preincubation with rhPlexin D1 (2 µg/mL) (right image). Nuclei are counterstained with DAPI (blue). (C) Immunostaining for IB4 (red), a marker of unmyelinated C-fiber type TG neurons, mostly colocalized with IgG (green) in a representative patient with IPTN (case 1). (D) Immunostaining for NFH (red), a marker of myelinated Aβ and Aδ fiber type TG neurons, did not colocalize with IgG binding (green) in case 1. (E) IFAs with the mouse brain, heart, and kidney using IgG from a representative patient with IPTN (case 1). IgG from case 1 (green) restrictedly bound to endothelial cells (white arrows) in the cerebral cortex (upper image), heart (middle image), and kidney (lower image). The weak immunostaining (green) of endothelial cells by IgG from case 1 was prevented by preincubation with rhPlexin D1 (2 µg/mL). Nuclei are counterstained with DAPI (blue). Scale bars: (A–E) = 50 µm. IB4 = isolectin B4; IFA = immunofluorescence assay; IPTN = idiopathic painful trigeminal neuropathy; NFH = neurofilament heavy chain; Plexin D1-IgG = anti-Plexin D1 antibody; rhPlexin D1 = recombinant human Plexin D1; TG = trigeminal ganglion.

Discussion

The main findings of the present study are as follows: (1) a small fraction of patients with IPTN had Plexin D1-IgG and (2) Plexin D1-IgG bound directly to small TG neurons, especially IB4-positive pain-conducting unmyelinated neurons.

Plexin D1 mRNA is present at low levels in various normal adult tissues.4 Recent studies found Plexin D1 mRNA and protein in normal adult human and mouse DRG2,5 but not in the normal adult human brain and heart.6 In our study, Plexin D1-IgG from patients with IPTN immunoreacted with DRG neurons but only weakly immunostained endothelial cells in the brain, heart, and kidney, which is largely consistent with previous findings. NP is attributable to preferential binding of Plexin D1-IgG to DRG neurons, although endothelial cell dysfunction in NP should also be carefully monitored. Transcriptome analyses of TG and DRG neurons revealed that Plexin D1 mRNA is significantly enriched in DRG compared with TG neurons.5 Therefore, limb and trunk pain may occur more frequently than facial pain in Plexin D1-IgG–positive cases.

IVIg was ineffective for pain relief in case 1, whereas a combined administration of corticosteroids and azathioprine improved pain in case 2. The difference in disease duration (2 months vs 15 years) may explain the inconsistent effects of immunotherapy because long disease duration is associated with neural damage in autoantibody-mediated neuropathy.7 Alternatively, Plexin D1-IgG–mediated disease may be resistant to IVIg because some subclasses of IgG autoantibodies are reported to show resistance to IVIg.8

A study limitation is that we did not establish a cell-based assay for Plexin D1-IgG because transfection of various cell types, including human embryonic kidney 293 cells, with plasmids encoding Plexin D1 induced apoptosis.9 Establishment of a reliable cell-based assay for Plexin D1-IgG requires further study.

In conclusion, we have demonstrated the presence of Plexin D1-IgG in a small fraction of patients with IPTN and Plexin D1-IgG reactivity toward small TG neurons, which indicates that Plexin D1-IgG–related NP could manifest as facial pain in addition to limb and trunk pain.

Study funding

This study was funded in part by grants from the Japan Society for the Promotion of Science (JSPS) KAKENHI (Grant Nos. 19H01045 and 19K17037).

Disclosure

T. Fujii is supported by grants from JSPS KAKENHI (Grant No. 19K17037), Mitsubishi Tanabe Pharma, Osoegawa Neurology Clinic, Bayer Yakuhin, Ltd., and the Japan Blood Products Organization and received speaker honoraria payments from the Takeda Pharmaceutical Company and Eisai. R. Yamasaki is supported by a grant from JSPS KAKENHI (Grant No. 19K07963). Y. Miyachi, K. Iinuma, and Y. Hashimoto report no disclosures. N. Isobe is supported by grants from Mitsubishi Tanabe Pharma, Osoegawa Neurology Clinic, Bayer Yakuhin, Ltd., and the Japan Blood Products Organization. T. Matsushita received speaker honoraria payments from Mitsubishi Tanabe Pharma, the Takeda Pharmaceutical Company, and Biogen Japan. J.-i. Kira is supported by grants from JSPS KAKENHI (Grant No. 19H01045) and Health and Labour Sciences Research Grants on Intractable Diseases [H29-Nanchitou (Nan)-Ippan-043] and received consultancy fees, speaking fees, and/or honoraria from Novartis Pharma, Mitsubishi Tanabe Pharma, Boehringer Ingelheim, Teijin Pharma, Takeda Pharmaceutical Company, Otsuka Pharmaceutical, Astellas Pharma, Pfizer Japan, and Eisai. Go to Neurology.org/NN for full disclosures.

Acknowledgment

The authors thank Jeremy Allen, PhD, from the Edanz Group (edanzediting.com/ac) for editing a draft of this manuscript.

Appendix Authors

Table
Table

Footnotes

  • Go to Neurology.org/NN for full disclosures. Funding information is provided at the end of the article.

  • The Article Processing Charge was funded by the authors.

  • Received March 24, 2020.
  • Accepted in final form May 14, 2020.
  • Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND), which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

References

  1. 1.↵
    Headache Classification Committee of the International Headache Society (IHS) The International Classification of Headache Disorders, 3rd edition. Cephalalgia 2018;38:1–211.
    OpenUrlCrossRefPubMed
  2. 2.↵
    1. Fujii T,
    2. Yamasaki R,
    3. Iiunuma K, et al
    . A novel autoantibody against Plexin D1 in patients with neuropathic pain. Ann Neurol 2018;84:208–224.
    OpenUrl
  3. 3.↵
    1. Prineas JW,
    2. Parratt JDE
    . Multiple sclerosis: serum anti-CNS autoantibodies. Mult Scler 2018;24:610–622.
    OpenUrl
  4. 4.↵
    1. Rehman M,
    2. Gurrapu S,
    3. Cagnoni G, et al
    . PlexinD1 is a novel transcriptional target and effector of notch signaling in cancer cells. PLoS One 2016;11:e0164660.
    OpenUrl
  5. 5.↵
    1. Megat S,
    2. Ray PR,
    3. Tavares-Ferreira D, et al
    . Differences between dorsal root and trigeminal ganglion nociceptors in mice revealed by translational profiling. J Neurosci 2019;39:6829–6847.
    OpenUrlAbstract/FREE Full Text
  6. 6.↵
    1. Roodink I,
    2. Verrijp K,
    3. Raats J, et al
    . Plexin D1 is ubiquitously expressed on tumor vessels and tumor cells in solid malignancies. BMC Cancer 2009;9:297.
    OpenUrlCrossRefPubMed
  7. 7.↵
    1. Ogata H,
    2. Yamasaki R,
    3. Hiwatashi A, et al
    . Characterization of IgG4 anti-neurofascin 155 antibody-positive polyneuropathy. Ann Clin Transl Neurol 2015;2:960–971.
    OpenUrl
  8. 8.↵
    1. Devaux JJ,
    2. Miura Y,
    3. Fukami Y, et al
    . Neurofascin-155 IgG4 in chronic inflammatory demyelinating polyneuropathy. Neurology 2016;86:800–807.
    OpenUrl
  9. 9.↵
    1. Luchino J,
    2. Hocine M,
    3. Amoureux MC, et al
    . Semaphorin 3E suppresses tumor cell death triggered by the Plexin D1 dependence receptor in metastatic breast cancers. Cancer Cell 2013;24:673–685.
    OpenUrlCrossRefPubMed

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Neurology: Neuroimmunology & Neuroinflammation
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