Article Figures & Data
Figures
- Figure 1 EV enrichment, MACSPlex exosome assay, and EV characterization
(A) Protocol for EV enrichment and MACSPlex exosome assay. Blood collected into anticoagulant EDTA tubes underwent serial centrifugation to eliminate cellular components and larger EVs. Plasma samples were incubated overnight with dye-labeled capture beads coated with antibodies against 37 different EV surface antigens. Detection antibodies against CD9, CD63, and CD81 were then added and incubated for 1 hour. After washing steps, samples were analyzed by flow cytometry. (B) Nanoparticle concentration (N/mL plasma) by nanoparticle tracking analysis (NTA), stratified for diameter (smaller nanoparticles, 30–150 nm; larger nanoparticles 151–500 nm). (C) Mean median fluorescence intensity (MFI) for CD9, CD63, and CD81 at flow cytometry analysis. (D) Correlation between mean MFI of CD9−CD63−CD81 and N/mL by NTA: the regression line is reported in red, with 95% CI. (E) Western blot of samples from HC, PD, MSA, and AP-Tau subjects after immunocapturing compared with whole plasma (dilution 1:100), showing the presence of specific EV markers (CD81, Alix, tumor susceptibility gene 101) and the absence of plasma contaminants (apolipoprotein A1, GRP94). Data are expressed as median and interquartile range; p values < 0.05 were considered significant (*p < 0.05, **p < 0.01, ***p < 0.001). AP-Tau = atypical parkinsonism with tauopathies; EV = extracellular vesicle; HC = healthy control; MSA = multiple system atrophy; PD = Parkinson disease.
- Figure 2 Differential expression of extracellular vesicle (EV)-surface markers
Patients' stratification for diagnosis and EV surface marker expression (expressed as normalized median fluorescence intensity [nMFI]). (A) Heatmap representation of the 17 EV surface markers differentially expressed between patients with PD, MSA, and AP-Tau and HCs (purple = low nMFI, yellow = high nMFI). (B) Canonical plot showing patients according to the diagnosis: PD, red vs MSA, orange vs AP-Tau, gray vs HC, blue; the model was built considering the 37 EV surface markers analyzed by flow cytometry. The axes of the plot (canonical 1, canonical 2, and canonical 3) were calculated from weighted linear combinations of variables to maximize separation between the 4 groups. Each subject is represented by a point, and spheres include patients with a linear combination coefficient that falls within the mean ± SD (canonical 1 ± SD; canonical 2 ± SD; canonical ± SD). AP-Tau = atypical parkinsonism with tauopathies; HC = healthy control; MSA = multiple system atrophy; PD = Parkinson disease.
- Figure 3 Extracellular vesicle (EV) surface proteins upregulated in PD, MSA, and AP-Tau and functional evaluation of their protein interactors
PPI network showing the first neighbors of each differentially expressed EV surface marker in (A) PD, (B) MSA, and (C) AP-Tau vs HC. (D) Kyoto Encyclopedia of Genes and Genomes pathways enriched by considering the first neighbors of each EV surface protein in PD, MSA, and AP-Tau vs HC; DAVID database background: Homo sapiens, gene count >5 and p < 0.001. AP-Tau = atypical parkinsonism with tauopathies; HC = healthy control; MSA = multiple system atrophy; PD = Parkinson disease; PPI = protein-protein interaction.
- Figure 4 Correlations between clinical scales and extracellular vesicle (EV) surface marker expression
Correlations between EV surface markers normalized MFI, nanoparticle concentration (N/mL plasma), and clinical parameters in patients with Parkinson disease (circles; A-D) and multiple system atrophy (triangles; E-G). The regression line is reported together with its 95% CI (dashed line). BDI-II = Beck Depression Inventory II; H&Y = Hoehn and Yahr scale; MFI = median fluorescence intensity; MMSE = Mini-Mental State Examination; MoCA = Montreal Cognitive Assessment; RBD = REM sleep behavior disorder screening questionnaire.
- Figure 5 Receiver operating characteristic (ROC) curve analysis of extracellular vesicle (EV)-surface markers
ROC curves identifying the best cutoff for each EV surface marker, discriminating pathologic groups from HC. The referral line is reported in gray. (A) PD vs HC; (B) MSA vs HC; (C) AP-Tau vs HC. In each plot, ROC curves for the combination of the 3 EV surface markers with the highest AUCs and for a compound EV marker (linear weighted combination of all EV surface markers differentially expressed for each comparison) are shown (black and red lines, respectively). The tables provide asymptotic significance AUC with 95% CI, sensitivity, and specificity on the compound EV markers. p Values < 0.05 were considered significant. AP-Tau = atypical parkinsonism with tauopathies; AUC = area under the curve; HC = healthy control; MSA = multiple system atrophy; PD = Parkinson disease.
- Figure 6 Random forest (RF) modeling to predict diagnosis and its validation in an external cohort of subjects
RF modeling to diagnose patients based on the combination of the 17 differentially expressed extracellular vesicle surface markers. (A) Representation of 1 of the 20 different classification trees created by the algorithm to predict the diagnosis PD vs MSA vs AP-Tau vs HC (B–H). Confusion matrix reporting real and predicted diagnosis, accuracy, sensitivity, specificity, and internal validation by the leave-one-out algorithm for each comparison (see Methods). (I) External validation of the RF model; 40 patients were included in the analysis (20 HC, blue; 10 PD, red; 5 MSA, orange; 5 AP-Tau, gray). AP-Tau = atypical parkinsonism with tauopathies; HC = healthy control; MSA = multiple system atrophy; PD = Parkinson disease.
Tables
Letters: Rapid online correspondence
REQUIREMENTS
You must ensure that your Disclosures have been updated within the previous six months. Please go to our Submission Site to add or update your Disclosure information.
Your co-authors must send a completed Publishing Agreement Form to Neurology Staff (not necessary for the lead/corresponding author as the form below will suffice) before you upload your comment.
If you are responding to a comment that was written about an article you originally authored:
You (and co-authors) do not need to fill out forms or check disclosures as author forms are still valid
and apply to letter.
Submission specifications:
- Submissions must be < 200 words with < 5 references. Reference 1 must be the article on which you are commenting.
- Submissions should not have more than 5 authors. (Exception: original author replies can include all original authors of the article)
- Submit only on articles published within 6 months of issue date.
- Do not be redundant. Read any comments already posted on the article prior to submission.
- Submitted comments are subject to editing and editor review prior to posting.
You May Also be Interested in
Topics Discussed
Alert Me
Recommended articles
Electrophysiologic patterns of oral-pharyngeal swallowing in parkinsonian syndromes
Network correlates of disease severity in multiple system atrophy
Characteristics of idiopathic REM sleep behavior disorder and that associated with MSA and PD
Early predictors of mortality in parkinsonism and Parkinson disease