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March 2021; 8 (2) ArticleOpen Access

CIDP Antibodies Target Junction Proteins and Identify Patient Subgroups

An Autoantigenomic Approach

View ORCID ProfileChristian P. Moritz, View ORCID ProfileYannick Tholance, View ORCID ProfileOda Stoevesandt, Karine Ferraud, View ORCID ProfileJean-Philippe Camdessanché, View ORCID ProfileJean-Christophe Antoine
First published January 6, 2021, DOI: https://doi.org/10.1212/NXI.0000000000000944
Christian P. Moritz
From the Department of Neurology (C.P.M., K.F., J.-P.C., J.-C.A.), and Department of Biochemistry (Y.T.), University Hospital of Saint-Etienne; Synaptopathies and Autoantibodies (C.P.M., Y.T., J.-P.C., J.-C.A.), Institut NeuroMyoGène, INSERM U1217/CNRS UMR 5310, University of Lyon, University Jean-Monnet, Saint-Étienne, France; and Cambridge Protein Arrays Ltd. (O.S.), Babraham Research Campus, United Kingdom.
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Yannick Tholance
From the Department of Neurology (C.P.M., K.F., J.-P.C., J.-C.A.), and Department of Biochemistry (Y.T.), University Hospital of Saint-Etienne; Synaptopathies and Autoantibodies (C.P.M., Y.T., J.-P.C., J.-C.A.), Institut NeuroMyoGène, INSERM U1217/CNRS UMR 5310, University of Lyon, University Jean-Monnet, Saint-Étienne, France; and Cambridge Protein Arrays Ltd. (O.S.), Babraham Research Campus, United Kingdom.
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Oda Stoevesandt
From the Department of Neurology (C.P.M., K.F., J.-P.C., J.-C.A.), and Department of Biochemistry (Y.T.), University Hospital of Saint-Etienne; Synaptopathies and Autoantibodies (C.P.M., Y.T., J.-P.C., J.-C.A.), Institut NeuroMyoGène, INSERM U1217/CNRS UMR 5310, University of Lyon, University Jean-Monnet, Saint-Étienne, France; and Cambridge Protein Arrays Ltd. (O.S.), Babraham Research Campus, United Kingdom.
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Karine Ferraud
From the Department of Neurology (C.P.M., K.F., J.-P.C., J.-C.A.), and Department of Biochemistry (Y.T.), University Hospital of Saint-Etienne; Synaptopathies and Autoantibodies (C.P.M., Y.T., J.-P.C., J.-C.A.), Institut NeuroMyoGène, INSERM U1217/CNRS UMR 5310, University of Lyon, University Jean-Monnet, Saint-Étienne, France; and Cambridge Protein Arrays Ltd. (O.S.), Babraham Research Campus, United Kingdom.
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Jean-Philippe Camdessanché
From the Department of Neurology (C.P.M., K.F., J.-P.C., J.-C.A.), and Department of Biochemistry (Y.T.), University Hospital of Saint-Etienne; Synaptopathies and Autoantibodies (C.P.M., Y.T., J.-P.C., J.-C.A.), Institut NeuroMyoGène, INSERM U1217/CNRS UMR 5310, University of Lyon, University Jean-Monnet, Saint-Étienne, France; and Cambridge Protein Arrays Ltd. (O.S.), Babraham Research Campus, United Kingdom.
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Jean-Christophe Antoine
From the Department of Neurology (C.P.M., K.F., J.-P.C., J.-C.A.), and Department of Biochemistry (Y.T.), University Hospital of Saint-Etienne; Synaptopathies and Autoantibodies (C.P.M., Y.T., J.-P.C., J.-C.A.), Institut NeuroMyoGène, INSERM U1217/CNRS UMR 5310, University of Lyon, University Jean-Monnet, Saint-Étienne, France; and Cambridge Protein Arrays Ltd. (O.S.), Babraham Research Campus, United Kingdom.
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CIDP Antibodies Target Junction Proteins and Identify Patient Subgroups
An Autoantigenomic Approach
Christian P. Moritz, Yannick Tholance, Oda Stoevesandt, Karine Ferraud, Jean-Philippe Camdessanché, Jean-Christophe Antoine
Neurol Neuroimmunol Neuroinflamm Mar 2021, 8 (2) e944; DOI: 10.1212/NXI.0000000000000944

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Abstract

Objective To discover systemic characteristics in the repertoires of targeted autoantigens in chronic inflammatory demyelinating polyneuropathy (CIDP), we detected the entire autoantigen repertoire of patients and controls and analyzed them systematically.

Methods We screened 43 human serum samples, of which 22 were from patients with CIDP, 12 from patients with other neuropathies, and 9 from healthy controls via HuProt Human Proteome microarrays testing about 16,000 distinct human bait proteins. Autoantigen repertoires were analyzed via bioinformatical autoantigenomic approaches: principal component analysis, analysis of the repertoire sizes in disease groups and clinical subgroups, and overrepresentation analyses using Gene Ontology and PantherDB.

Results The autoantigen repertoires enabled the identification of a subgroup of 10/22 patients with CIDP with a younger age at onset and a higher frequency of mixed motor and sensory CIDP. IV immunoglobulin therapy responders targeted 3 times more autoantigens than nonresponders. No CIDP-specific autoantibody is present in all patients; however, anchoring junction components were significantly targeted by 86.4% of patients with CIDP. There are potential novel CIDP-specific autoantigens such as the myelination- or axo-glial structure–related proteins actin-related protein 2/3 complex subunit 1B, band 4.1-like protein 2, cadherin-15, cytohesin-1, epidermal growth factor receptor, ezrin, and radixin.

Conclusions The repertoire of targeted autoantigens of patients with CIDP differs in a systematic degree from those of controls. Systematic autoantigenomic approaches can help to understand the disease and to discover novel bioinformatical tools and novel autoantigen panels to improve diagnosis, treatment, prognosis, or patient stratification.

Glossary

CIDP=
chronic inflammatory demyelinating polyneuropathy;
ENMG=
electroneuromyography;
FDR=
false discovery rate;
GO=
Gene Ontology;
HC=
healthy control;
IVIg=
IV immunoglobulin;
mRS=
modified Rankin score;
ONP=
other peripheral neuropathies;
PC=
principal component;
PCA=
principal component analysis;
PNS=
peripheral nervous system;
R2=
coefficient of determination;
SjS=
Sjögren syndrome

Chronic inflammatory demyelinating polyneuropathy (CIDP) is a rare disease of the peripheral nervous system that is considered to be immune mediated.1,2 Pathogenetic antibodies directed to proteins of the region of the node of Ranvier permit the identification of a small subgroup of patients (<10% of CIDP) with particular features and therapeutic responses.3,–,5 However, in the majority of patients, no specific antibodies have been identified so far. This may be due to unsuitable identification methods or due to inappropriate hypotheses of one main antigen being targeted by the immune system. Indeed, the immune response is probably a complex process involving several antibodies and several targets with different functions in the development of the immune response and its regulation. In this case, a systemic approach may lead to a better understanding of immune-mediated diseases.6,–,9 Hence, recent methods aiming at identifying and understanding the entire repertoire of targeted autoantigens via autoantigenomics have been developed for the study of the immune response.10 Thus, instead of focusing on single autoantigens, this study takes an autoantigenomic approach pointing to the entire autoantigen repertoire in a systematic way via bioinformatical tools. To do so, sera from 43 subjects were tested with HuProt 3.1 Human Proteome arrays containing 15,798 human proteins expressed in yeast, representing about 75% of the gene-centric human proteome.

Methods

Standard Protocol Approvals, Registrations, and Patient Consents

The retrospective case-control and observational study involves the use of sera from human subjects, was approved by the ethical committee of the University Hospital of Saint-Etienne, France, and has been performed in accordance with the Code of Ethics of the World Medical Association (the Declaration of Helsinki). All participants provided written informed consent. The privacy rights of human subjects were observed. No animal experiments were conducted for this study.

Subject Selection, Description of Population, and Serum Preparation

We collected serum samples from 22 patients with definite CIDP according to the European Federation of Neurological Societies/Peripheral Nerve Society guidelines.11

Selection criteria were the following: definite form of CIDP,11 age ≥18 years, and absence of severe secondary axonal degeneration according to the electroneuromyography (ENMG). Definite CIDP was either typical (typical clinical presentation, typical ENMG data) or atypical CIDP (atypical clinical presentation, typical ENMG data).11,12 The choice of treatment was at the discretion of the neurologist who cared for the patients.

As controls, we selected roughly age and sex-matched sera samples from 12 patients with other peripheral neuropathies (ONP), 11 of whom with an associated autoimmune context—7 Sjögren syndrome (SjS), 1 autoimmune hepatitis, 1 sarcoidosis, 1 systemic lupus erythematosus, and 1 undifferentiated connective tissue disease—and 9 healthy controls (HCs) originating from the blood donation service of the French Blood Establishment in Saint-Étienne, France. All samples were selected retrospectively. Sera were prepared and stored as previously described.13

Clinical Data

For all subjects, we obtained demographic data such as age at sampling date and sex. For patients with CIDP, the following clinical data were obtained in addition: age at disease onset, course of the disease, type and topography of neurologic symptoms, type of disease's progression (chronic evolution with or without relapse or only relapses), modified Rankin scores (mRSs) during the disease course,14 ENMG data,15 biological data (CSF proteins or monoclonal gammopathy), presence of ataxia, presence of pain, concerned nerve type (sensory or motor), onset delay (acute ≤2 months, subacute = 2–6 months, or chronic ≥6 months),12 and IV immunoglobulin (IVIg) response defined as an increase of the INCAT score by ≥1 after a treatment period of 3 months.16 Because of the low number of patients treated with other immunomodulatory treatment, we did not address their response. For the comparison of IVIg responders vs nonresponders, we tested for biasing confounder effects (sampling age, delay between disease onset and sampling date, comorbidities, monoclonal gammopathy, clinical severity before treatment, and clinical presentation).

Protein Microarrays and Definition of Repertoires

Sera were tested on HuProt 3.1 Human Proteome microarrays (CDI Laboratories, Baltimore, MD) as described previously.17 Most of the proteins, 14,870 (94.1%) in numbers, are full-length proteins. The full lists of resulting group-specific antigen repertoires were applied for the set of bioinformatical methods described below, aiming at a systemic understanding.

Principal Component Analyses

To compare the autoantigen repertoires of CIDP and ONP + HC, principal component analysis (PCA) was performed with the software tool from The Institute for Genomic Research, multiple experimental viewer (tigr.org/software/tm4/mev.html). PCA analysis was performed with the combined sets of intra-z values of the 3 study group-specific repertoires to identify systemic differences between them and to explore the repertoire in CIDP for potential subgroups.

Number of Targeted Antigens Per Subject

For each patient, the number of targeted antigens was counted if both of the following criteria were fulfilled: (1) intra-z score ≥2.5 and (2) inter-z score ≥4. For each study group, the corresponding other 2 study groups were used as the basis for calculating mean and SD.17

Panther Analysis: Gene Ontology Overrepresentation Test

PANTHER online software (pantherdb.org/) was applied to identify the Gene Ontology (GO) Cellular Component categories covered by the repertoires of targeted antigens (described in more detail in e-Methods, links.lww.com/NXI/A385). In the first selection steps, we selected only categories that fulfilled all of the following categories: (1) contain ≥3 targeted proteins; (2) whose number of targeted proteins is ≥5× higher for 1 of the 3 groups compared with the corresponding other 2; and (3) cover ≥4% of the CIDP or ONP repertoires. For the analysis based on the group-specific antigen repertoires, the percentage represents the frequency of targeted proteins set into relation with the repertoire size. Statistical analyses selected the categories that were significantly overrepresented in the CIDP repertoire compared with the HC repertoire.

Statistics

To show the statistical dispersion, we used the median with the 25th and 75th percentiles (abbreviated Q1–Q3 in the text) or the mean with 95% CI. For the nonparametric hypothesis tests (comparison of numbers of targeted antigens per patient), we used the Wilcoxon-Mann-Whitney test (1-sided for H1: a<b, i.e., study groups and IVIg response in the comparison of antigen numbers; 2-sided for H1: a≠b, i.e., all other comparisons) via the online tool available at ccb-compute2.cs.uni-saarland.de/wtest/ (comparison performed on October 19, 2018).18 p Value ≤0.05 was considered positive after Benjamini-Hochberg correction19 at level 0.05.

For the comparison of clinical data between subgroups of patients with CIDP, we also used the Wilcoxon-Mann-Whitney test, and the difference of median groups was estimated by calculating the Hodges-Lehmann median with the corresponding 95% CIs.

To compare frequencies of GO categorical data, the Fisher exact test was applied via the online tool available at langsrud.com/stat/fisher.htm.20 p Value ≤0.05 was considered positive after Benjamini-Hochberg correction at level 0.05. For the comparison of clinical categorial data between subgroups of patients with CIDP, we used the χ2 test, and the difference of frequencies with the corresponding 95% CI was calculated. Correlation analysis was performed using coefficients of determination (R2) in Excel (version 1906; Microsoft Office ProPlus). Missing data were excluded from the analyses.

Data Availability

All anonymized data from this study or all related documents will be shared by request from any qualified investigator.

Results

Patients

Of the 22 patients with CIDP, 15 were males and 7 were females with a median age of 65.2 years (Q1–Q3: 58.6–71.3). Thirteen patients had a chronic course, 6 had a chronic course with relapses, and 3 had a relapsing course. Twenty patients received IVIg, and 15 of 19 were considered responders (1 missing value). In 19 patients, IVIg was used alone, and 1 patient received steroids, and IVIg for 1 month followed by IVIg alone for 22 months. In this patient, response to IVIg was assessed under IVIg alone, at least 3 months after the end of steroids. Other immunomodulatory or immunosuppressive treatments were also used in 8 patients prior or after IVIg treatment (corticosteroids, azathioprine, or plasma exchanges). In 2 patients, corticosteroids were used alone. The serum sample was obtained before any treatment in 13 of 18 patients (without a significant difference between the IVIg responders and nonresponders, p = 0.17). Five sera of 18 were sampled in patients who already had only 1 session of treatment in the past (pretreated); all these patients were IVIg responders (median sampling delay after the last immunomodulatory treatment: 42 days (Q1–Q3: 37–79 days; extreme values: 20–450 days).

Of the 21 control subjects (12 with ONP and 9 HC), 11 were males and 10 were females with a median age of 59.7 years (Q1–Q3: 54.2–65.0).

Group-Specific Repertoires of Targeted Antigens

The selection of group-specific targeted antigens (antigens recognized only by the patients of a given group) resulted in 3 repertoires comprising 716 CIDP-specific antigens, 226 ONP-specific antigens, and 159 HC-specific antigens. Among them, 180 (25%) CIDP-specific, 30 (13%) ONP-specific, and 11 (7%) HC-specific antigens were shared by at least 2 subjects. There was no significant difference between the CIDP, ONP, and HC groups regarding the mean number of antigens recognized by each subject: CIDP: 19 (CI: 13.6–24.3), ONP: 14 (CI: 10.0–17.1), and HC: 13 (CI: 7.4–18.8; figure 1A).

Figure 1
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Figure 1 Number of Targeted Antigens Among Sample Groups

(A and B) Primary comparisons concerning the 3 sample groups (CIDP, ONP, and HC) and IVIg response among CIDP. (C–N) Comparison of further CIDP subgroups based on clinical and personal data. Spots exhibit the number of targeted antigens per subject. Bar diagram shows corresponding means of the group; error bars show SEM. *p ≤ 0.05, Wilcoxon-Mann-Whitney test, FDR ≤0.05. CIDP = chronic inflammatory demyelinating polyneuropathy; FDR = false discovery rate; HC = healthy control; max mRS = maximal modified Rankin score; ONP = other peripheral neuropathies; n.s. = not significant; R2 = coefficient of determination.

No antigen was specifically recognized by all patients with CIDP (i.e., not recognized by any of the control sera), and the number of patients specifically reacting with 1 shared antigen varied from 2 to 8. As a quality control for the HuProt 3.1 protein arrays, we used the identification by the array of well-characterized autoantibodies detected by routine antibody-screening of sera for organ and non–organ-specific antibodies. Thus, gastritis autoantibody against the plasma membrane protein H+/K+-ATPase in a patient with CIDP and anti-SSA1 and SSA2 antibodies in 3 patients with OND and SjS syndrome were identified by the protein array.

The Repertoire of Targeted Antigens Identifies Subgroups of Patients With CIDP

The variation explained by the first 3 principal components (PCs) of the PCA was 23% (PC1 = 10%, PC2 = 8%, and PC3 = 5%), indicating a mild general discrimination of the 3 study groups. A subgroup of 10 patients with CIDP clustered (PCA cluster 1, blue ellipse, figure 2, A and B) apart from the ONP, HC, and the remaining 12 CIDP samples (PCA cluster 2, red ellipse, figure 2, A and B) when plotting PC2 and PC3, suggesting that these patients had a specific autoantigenome.

Figure 2
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Figure 2 PCA of Autoantigen Repertoires in CIDP and ONP + HC Groups

PCA in a 2D projection spanned by 2 principal components (PC) for all autoantigens for each subject of patients with CIDP (blue squares), ONP (red squares), and HC (green squares). (A) PC1 and PC2. (B) PC1 and PC3. The variation explained by the PC is indicated in parentheses. Ellipses distinguish a CIDP subgroup (cluster 1, blue ellipse) that clusters apart from ONP + HC and another CIDP subgroup that does not (cluster 2, red ellipse). CIDP = chronic inflammatory demyelinating polyneuropathy; HC = healthy control; ONP = other peripheral neuropathies; PCA = principal component analysis.

In the univariate model, clinical data of PCA clusters 1 and 2 were compared (table e-1, links.lww.com/NXI/A385). A younger age at onset and a higher frequency of mixed motor and sensory form of CIDP were associated with patients with CIDP of PCA cluster 1.

More Antigens Targeted in IVIg Responders

Among CIDP, the number of reactive antigens was not correlated with age at onset and sampling, sex, CIDP subtype, course, pain, ataxia, maximal mRS, axonal loss or conduction blocks on ENMG, or serum-storing duration (figure 1, C–N).

However, we found on average 3 times more reactive antigens in IVIg responders compared with nonresponders (responders' mean: 22; CI: 15.9–28.9; nonresponders' mean: 8; CI: 2.9–12.1; p = 0.01, false discovery rate ≤0.05; figure 1B). To exclude potential confounder effects, we compared IVIg responders vs nonresponders and found no differences concerning sampling age, delay between disease onset and sampling, presence of comorbidities, monoclonal gammopathy, clinical severity before treatment, clinical presentation, and electrophysiologic data, apart from the presence of conduction blocks, which was more frequent in IVIg responders compared with nonresponders (table e-2, links.lww.com/NXI/A385). The presence of the 5 pretreated patients in the group of IVIg responders was not a confounding factor as there was no significant difference regarding the numbers of targeted antigens between the IVIg-naive and pretreated patients (20 [CI: 13.2–26.7] vs 15 [CI: 10.3–20.5]; p = 0.31), showing that the 5 pretreated patients did not influence the significant difference of the number of targeted antigens between IVIg responders and nonresponders.

Anchoring Junction Proteins Were Significantly Targeted by Sera of Patients With CIDP

Two hundred seventy-five (38.4%) antigens among the CIDP repertoire, 79 (35.0%) antigens among the ONP repertoire, and 61 (38.4%) antigens among the HC repertoire were annotated to at least one of the cellular components, “Plasma membrane” and/or “Extracellular space” according to GO.

The overrepresentation analysis of cellular components with the Panther algorithm showed that the category “anchoring junction” was significantly overrepresented in the CIDP repertoire compared with the HC repertoire (figure 3A). Nineteen of 22 patients with CIDP (86.4%) had antibodies against at least one of these proteins. In average, each of the detected anchoring junction proteins was targeted by 3.6 (CI: 2.2–5.0) patients with CIDP, which is significantly more than for each of the rest of CIDP-specific non–junction proteins being targeted by an average of 2.2 patients each (CI: 2.0–2.3; difference of mean 1.4 [CI 0.002–2.8]; figure 3B). Twenty-one of 35 (60.0%) targeted anchoring junction proteins were annotated to the cellular components “Plasma membrane” (15/35, 42.9%) and/or “Extracellular region or secreted” (16/35, 45.7%) according to GO and are listed in table 1. Although expressed in a wide range of organs, most of them (19/21) are known to play roles in the nervous system or even more specifically in the peripheral nervous system (13/21). Seven of them are known to play roles in myelination or in the organization or maintenance of axo-glial structures (actin-related protein 2/3 complex subunit 1B, band 4.1-like protein 2, cadherin-15, cytohesin-1, epidermal growth factor receptor, ezrin, and radixin). Each of the CIDP-specific anchoring junction protein was targeted by 1–6 patients, and each patient reacted with 0–14 of junction proteins.

Figure 3
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Figure 3 Overrepresentation Analyses of the Antigen Repertoires and Frequency of Patients Targeting Anchoring Junction Proteins

(A) Overrepresentation analyses of the antigen repertoires using the Cellular Component database of Gene Ontology via PantherDB. Percentages are calculated relative to the size of the respective antigen repertoires. **p ≤ 0.01, Fisher exact test. (B) Mean number of reactive patients (intra-z > 2) per antigen. *Exclusion of 0 by the confident interval of the mean difference (1.4; CI: 0.002–2.8); error bars: 95% CI.

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Table 1

Anchoring Junction Proteins Associated With the Plasma Membrane or Extracellular Space

Discussion

Autoantigenomics provides the opportunity to mine systemic comprehension from autoantibody repertoires.10 Using bioinformatical tools known from proteomics,21,22 this method seeks to discover significant patterns in the repertoire of targeted autoantigens. In other words, instead of the classical approach of searching 1 single autoantibody and defining the vast rest as noise, autoantigenomics is focusing on related sets of autoantigen groups that emerge from the data set. In this study, we implemented this systemic mining for 716 CIDP-specific antigens, resulting from a quasi-proteome-wide screening of 22 patients with definite CIDP. Although seemingly low, our sample sizes are in the same range as those of similar protein microarray studies.23,–,25

Our approach resulted in the following main conclusions. On a systematic level, (1) the recognized repertoire of targeted antigens enables the identification of patient subgroups with differing clinical patterns and responses to IVIg; (2) anchoring junction proteins are a significant target of the CIDP-specific antibody repertoire; and (3) on a single antigen level, our approach revealed several novel interesting autoantigens that could be embarked on by the community.

The antigen repertoire specific to CIDP identified in this study contains hundreds of proteins of which only 25% were recognized by several patients, showing that the greater part of the antibody response is individual and fingerprint-like.26 We found no specific antigen recognized by all the patients but groups of antigens that are targeted by several patients. This suggests that there is low chance of identifying 1 biomarker antibody for typical CIDP, even if the panel of antigens used in the study does not cover the totality, but only 75% of the human proteome, leaving open the possibility that this particular antigen is by chance in the missing part of the antigenome or spotted in a nonreactive conformation.

Instead, we found a complex antibody response. Of interest, this global antibody response was not produced haphazardly as it is correlated with different clinical aspects of the CIDP. Thus, using the CIDP-specific antigenome, PCA identified a cluster of 10/22 (45%) deviant patients who were younger at disease onset and more often had a mixed motor and sensory form of CIDP. Furthermore, patients who were IVIg responders targeted on average 3 times more antigens than IVIg nonresponders, and this was an independent effect. All these results, and especially those concerning the response to IVIg, were obtained with a low number of patients and need to be confirmed on a larger series. However, they are in keeping with the fact that CIDP is a heterogenous entity27 and that response to IVIg in this disease probably depends on complex immunologic factors specific to subgroups of patients.28 The identification of these subgroups may be helpful for treatment management and patient stratification in clinical trials.

An interesting finding is that anchoring junction proteins, although in a numerical minority in the human antigenome, are overrepresented in the repertoire of targeted antigens of CIDP. This was a general phenomenon since 86.4% of the patients with CIDP had antibodies against at least one of these proteins. The fact that each identified anchoring junction protein was on average targeted by significantly more patients with CIDP than all other CIDP-specific antigens also suggests that this is not an incidental antigen set. Anchoring junction proteins are important for maintaining the neuronal–glial cell shapes. Most of our detected anchoring junction proteins (14/21) interfere in some way with the actin cytoskeleton; 7 are known to play a role in the myelination or axo-glial structuring process (table 1). In addition, given their roles or locations in the peripheral nervous system, 3 of these proteins (CD59 glycoprotein, Ezrin, and Radixin) have previously been discussed as potential targets for autoantibodies in demyelinating neuropathies.29,30

Other junction31,–,33 or cell adhesion34,–,37 proteins of the nodal and paranodal region including contactin-1 and neurofascin 155 and 186 have been identified as antibody targets in subgroups of CIDP, confirming that these protein groups comprise important autoantigens in CIDP. However, in contrast to the predominantly neural proteins contactin-1 and neurofascin 155 and 186, the proteins identified in our study are—despite their roles in the nervous system—mostly widely expressed. Contactin-1 and neurofascin 155 and 186 are not on the HuProt 3.1 array. None of the 7 node of Ranvier proteins spotted on the arrays were targeted by the patients' sera. Thus, proteins specific to the node of Ranvier are probably not the main targets of antibodies in CIDP as a whole but only in a subgroup of patients who had a specific form of CIDP or even form another disease entity (e.g., [para-]nodopathy).38

Because of the low number of only 3 anti–junction protein–negative patients, it was not possible to determine whether a specific clinical pattern is associated with the targeting of these proteins. Antibodies reacting with the nodal and paranodal regions are probably causally involved in the lesioning process by interfering with their target.5,39,40 Whether this is the case with the antibodies identified in this study is not yet determined. Although the complex specific antibody response is linked with clinical characteristics of the CIDP, it is not possible to know whether it contributes to the lesioning process or is a secondary phenomenon or a mixture of both. Regarding a diagnostic potential, each of the antibodies identifies a restricted proportion of patients, as do the known antibodies reacting with the node of Ranvier. However, several of them, alone or in combination, may be candidate biomarkers for the diagnosis of CIDP or for managing the treatment by IVIg.

In conclusion, this article describes the application of autoantigenomics, i.e., the systematic analysis of the whole autoantigen repertoire, in the neurology field. The identified candidates present novel potential antibody targets of CIDP that could be embarked on—either as single antigens or panels—by the community. The discovered set of antibodies against anchoring junction proteins may be of interest for diagnostics, prognosis, and patient stratification. At the same time, the research community interested in not only CIDP but also other inflammatory neuropathies might broaden their view from single candidates to a more systemic view of antigen repertoires. Functional or compartmental sets of targeted antigens suggest novel, more systematic tools to diagnose and understand autoimmune neuropathies.

Study Funding

The study was funded, but not the article. University Hospital of Saint-Etienne; German Research Foundation (DFG; MO 3240/1-1:1); Association Française contre les Myopathies (AFM-MyoNeurALP project 6.1.1); BETPSY project, Investissements d'Avenir program of the French National Research Agency (ANR, reference ANR-18-RHUS-0012); and Fonds de dotation CSL Behring pour la recherche.

Disclosure

C.P. Moritz received travel grants from CSL Behring (France), outside the submitted work. Y. Tholance reports personal fees from The Binding Site and Alexion, outside the submitted work. O. Stoevesandt was an employee of Cambridge Protein Arrays Ltd. K. Ferraud declares no competing interests. J.-P. Camdessanché reports personal fees for lectures, consulting, writing of articles, or training courses from Akcea, Alnylam, Biogen, CSL Behring, Genzyme, Laboratoire Français des Biotechnologies (LFB), Merck, Novartis, Pfizer, Pharmalliance, Teva, Editions Scientifiques L&C, Edimark, Expression Santé, Natus, Scien, and SNF-Floerger, outside the submitted work, and is holding a patent on anti-FGFR3 autoantibodies. J.-C. Antoine received honoraria for scientific counseling from Pfizer and from a license on diagnostic test for the detection of anti-CRMP5 antibodies and travel grants from LFB, outside the submitted work, and is holding a patent on anti-FGFR3 autoantibodies. Go to Neurology.org/NN for full disclosures.

Acknowledgment

The authors thank Michael J. Taussig for hosting C.P.M. in his labs and for helpful discussions, Claudia Fonseca for technical assistance and teaching the handling with HuProt Human Proteome microarrays, Carole Rosier for contributing to diagnosing patients with CIDP, Evelyne Reynaud-Federspiel for helping with logistics regarding storage and sending of sera, Jérôme Honnorat for helpful discussions, Priyanka Garg for proofreading an earlier version of the manuscript, and all anonymous patients and donors for their contribution to this study. Some authors are members of the ERN Euro-NMD.

Appendix Authors

Table2

Footnotes

  • Go to Neurology.org/NN for full disclosures. Funding information is provided at the end of the article.

  • ↵* These authors contributed equally to this work and are the co–first authors.

  • The Article Processing Charge was funded by the authors.

  • Received April 11, 2020.
  • Accepted in final form October 29, 2020.
  • Copyright © 2021 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND), which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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  • All Immunology
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  • Chronic inflammatory demyelinating polyneuropathy

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