PT - JOURNAL ARTICLE AU - Reindl, Markus AU - Schanda, Kathrin AU - Woodhall, Mark AU - Tea, Fiona AU - Ramanathan, Sudarshini AU - Sagen, Jessica AU - Fryer, James P. AU - Mills, John AU - Teegen, Bianca AU - Mindorf, Swantje AU - Ritter, Nora AU - Krummrei, Ulrike AU - Stöcker, Winfried AU - Eggert, Juliane AU - Flanagan, Eoin P. AU - Ramberger, Melanie AU - Hegen, Harald AU - Rostasy, Kevin AU - Berger, Thomas AU - Leite, Maria Isabel AU - Palace, Jacqueline AU - Irani, Sarosh R. AU - Dale, Russell C. AU - Probst, Christian AU - Probst, Monika AU - Brilot, Fabienne AU - Pittock, Sean J. AU - Waters, Patrick TI - International multicenter examination of MOG antibody assays AID - 10.1212/NXI.0000000000000674 DP - 2020 Mar 05 TA - Neurology - Neuroimmunology Neuroinflammation PG - e674 VI - 7 IP - 2 4099 - http://nn.neurology.org/content/7/2/e674.short 4100 - http://nn.neurology.org/content/7/2/e674.full SO - Neurol Neuroimmunol Neuroinflamm2020 Mar 05; 7 AB - Objective To compare the reproducibility of 11 antibody assays for immunoglobulin (Ig) G and IgM myelin oligodendrocyte glycoprotein antibodies (MOG-IgG and MOG-IgM) from 5 international centers.Methods The following samples were analyzed: MOG-IgG clearly positive sera (n = 39), MOG-IgG low positive sera (n = 39), borderline negative sera (n = 13), clearly negative sera (n = 40), and healthy blood donors (n = 30). As technical controls, 18 replicates (9 MOG-IgG positive and 9 negative) were included. All samples and controls were recoded, aliquoted, and distributed to the 5 testing centers, which performed the following antibody assays: 5 live and 1 fixed immunofluorescence cell-based assays (CBA-IF, 5 MOG-IgG, and 1 MOG-IgM), 3 live flow cytometry cell-based assays (CBA-FACS, all MOG-IgG), and 2 ELISAs (both MOG-IgG).Results We found excellent agreement (96%) between the live CBAs for MOG-IgG for samples previously identified as clearly positive or negative from 4 different national testing centers. The agreement was lower with fixed CBA-IF (90%), and the ELISA showed no concordance with CBAs for detection of human MOG-IgG. All CBAs showed excellent interassay reproducibility. The agreement of MOG-IgG CBAs for borderline negative (77%) and particularly low positive (33%) samples was less good. Finally, most samples from healthy blood donors (97%) were negative for MOG-IgG in all CBAs.Conclusions Live MOG-IgG CBAs showed excellent agreement for high positive and negative samples at 3 international testing centers. Low positive samples were more frequently discordant than in a similar comparison of aquaporin-4 antibody assays. Further research is needed to improve international standardization for clinical care.ADEM=acute disseminated encephalomyelitis; AQP4=aquaporin-4; CBA=cell-based assay; FACS=fluorescence-activated cell sorting; IF=immunofluorescence; IfQ=Institute for Quality Assurance; Ig=immunoglobulin; MOG=myelin oligodendrocyte glycoprotein; NMOSD=neuromyelitis optica spectrum disorder