RT Journal Article SR Electronic T1 International multicenter examination of MOG antibody assays JF Neurology - Neuroimmunology Neuroinflammation JO Neurol Neuroimmunol Neuroinflamm FD Lippincott Williams & Wilkins SP e674 DO 10.1212/NXI.0000000000000674 VO 7 IS 2 A1 Reindl, Markus A1 Schanda, Kathrin A1 Woodhall, Mark A1 Tea, Fiona A1 Ramanathan, Sudarshini A1 Sagen, Jessica A1 Fryer, James P. A1 Mills, John A1 Teegen, Bianca A1 Mindorf, Swantje A1 Ritter, Nora A1 Krummrei, Ulrike A1 Stöcker, Winfried A1 Eggert, Juliane A1 Flanagan, Eoin P. A1 Ramberger, Melanie A1 Hegen, Harald A1 Rostasy, Kevin A1 Berger, Thomas A1 Leite, Maria Isabel A1 Palace, Jacqueline A1 Irani, Sarosh R. A1 Dale, Russell C. A1 Probst, Christian A1 Probst, Monika A1 Brilot, Fabienne A1 Pittock, Sean J. A1 Waters, Patrick YR 2020 UL http://nn.neurology.org/content/7/2/e674.abstract AB Objective To compare the reproducibility of 11 antibody assays for immunoglobulin (Ig) G and IgM myelin oligodendrocyte glycoprotein antibodies (MOG-IgG and MOG-IgM) from 5 international centers.Methods The following samples were analyzed: MOG-IgG clearly positive sera (n = 39), MOG-IgG low positive sera (n = 39), borderline negative sera (n = 13), clearly negative sera (n = 40), and healthy blood donors (n = 30). As technical controls, 18 replicates (9 MOG-IgG positive and 9 negative) were included. All samples and controls were recoded, aliquoted, and distributed to the 5 testing centers, which performed the following antibody assays: 5 live and 1 fixed immunofluorescence cell-based assays (CBA-IF, 5 MOG-IgG, and 1 MOG-IgM), 3 live flow cytometry cell-based assays (CBA-FACS, all MOG-IgG), and 2 ELISAs (both MOG-IgG).Results We found excellent agreement (96%) between the live CBAs for MOG-IgG for samples previously identified as clearly positive or negative from 4 different national testing centers. The agreement was lower with fixed CBA-IF (90%), and the ELISA showed no concordance with CBAs for detection of human MOG-IgG. All CBAs showed excellent interassay reproducibility. The agreement of MOG-IgG CBAs for borderline negative (77%) and particularly low positive (33%) samples was less good. Finally, most samples from healthy blood donors (97%) were negative for MOG-IgG in all CBAs.Conclusions Live MOG-IgG CBAs showed excellent agreement for high positive and negative samples at 3 international testing centers. Low positive samples were more frequently discordant than in a similar comparison of aquaporin-4 antibody assays. Further research is needed to improve international standardization for clinical care.ADEM=acute disseminated encephalomyelitis; AQP4=aquaporin-4; CBA=cell-based assay; FACS=fluorescence-activated cell sorting; IF=immunofluorescence; IfQ=Institute for Quality Assurance; Ig=immunoglobulin; MOG=myelin oligodendrocyte glycoprotein; NMOSD=neuromyelitis optica spectrum disorder