RT Journal Article
SR Electronic
T1 International multicenter examination of MOG antibody assays
JF Neurology - Neuroimmunology Neuroinflammation
JO Neurol Neuroimmunol Neuroinflamm
FD Lippincott Williams & Wilkins
SP e674
DO 10.1212/NXI.0000000000000674
VO 7
IS 2
A1 Reindl, Markus
A1 Schanda, Kathrin
A1 Woodhall, Mark
A1 Tea, Fiona
A1 Ramanathan, Sudarshini
A1 Sagen, Jessica
A1 Fryer, James P.
A1 Mills, John
A1 Teegen, Bianca
A1 Mindorf, Swantje
A1 Ritter, Nora
A1 Krummrei, Ulrike
A1 Stöcker, Winfried
A1 Eggert, Juliane
A1 Flanagan, Eoin P.
A1 Ramberger, Melanie
A1 Hegen, Harald
A1 Rostasy, Kevin
A1 Berger, Thomas
A1 Leite, Maria Isabel
A1 Palace, Jacqueline
A1 Irani, Sarosh R.
A1 Dale, Russell C.
A1 Probst, Christian
A1 Probst, Monika
A1 Brilot, Fabienne
A1 Pittock, Sean J.
A1 Waters, Patrick
YR 2020
UL http://nn.neurology.org/content/7/2/e674.abstract
AB Objective To compare the reproducibility of 11 antibody assays for immunoglobulin (Ig) G and IgM myelin oligodendrocyte glycoprotein antibodies (MOG-IgG and MOG-IgM) from 5 international centers.Methods The following samples were analyzed: MOG-IgG clearly positive sera (n = 39), MOG-IgG low positive sera (n = 39), borderline negative sera (n = 13), clearly negative sera (n = 40), and healthy blood donors (n = 30). As technical controls, 18 replicates (9 MOG-IgG positive and 9 negative) were included. All samples and controls were recoded, aliquoted, and distributed to the 5 testing centers, which performed the following antibody assays: 5 live and 1 fixed immunofluorescence cell-based assays (CBA-IF, 5 MOG-IgG, and 1 MOG-IgM), 3 live flow cytometry cell-based assays (CBA-FACS, all MOG-IgG), and 2 ELISAs (both MOG-IgG).Results We found excellent agreement (96%) between the live CBAs for MOG-IgG for samples previously identified as clearly positive or negative from 4 different national testing centers. The agreement was lower with fixed CBA-IF (90%), and the ELISA showed no concordance with CBAs for detection of human MOG-IgG. All CBAs showed excellent interassay reproducibility. The agreement of MOG-IgG CBAs for borderline negative (77%) and particularly low positive (33%) samples was less good. Finally, most samples from healthy blood donors (97%) were negative for MOG-IgG in all CBAs.Conclusions Live MOG-IgG CBAs showed excellent agreement for high positive and negative samples at 3 international testing centers. Low positive samples were more frequently discordant than in a similar comparison of aquaporin-4 antibody assays. Further research is needed to improve international standardization for clinical care.ADEM=acute disseminated encephalomyelitis; AQP4=aquaporin-4; CBA=cell-based assay; FACS=fluorescence-activated cell sorting; IF=immunofluorescence; IfQ=Institute for Quality Assurance; Ig=immunoglobulin; MOG=myelin oligodendrocyte glycoprotein; NMOSD=neuromyelitis optica spectrum disorder