PT  - JOURNAL ARTICLE
AU  - Häusler, Darius
AU  - Hajiyeva, Zivar
AU  - Traub, Jan W.
AU  - Zamvil, Scott S.
AU  - Lalive, Patrice H.
AU  - Brück, Wolfgang
AU  - Weber, Martin S.
TI  - Glatiramer acetate immune modulates B-cell antigen presentation in treatment of MS
AID  - 10.1212/NXI.0000000000000698
DP  - 2020 May 01
TA  - Neurology - Neuroimmunology Neuroinflammation
PG  - e698
VI  - 7
IP  - 3
4099  - http://nn.neurology.org/content/7/3/e698.short
4100  - http://nn.neurology.org/content/7/3/e698.full
SO  - Neurol Neuroimmunol Neuroinflamm2020 May 01; 7
AB  - Objective We examined the effect of glatiramer acetate (GA) on B-cell maturation, differentiation, and antigen presentation in MS and experimental autoimmune encephalomyelitis (EAE).Methods A cross-sectional study of blood samples from 20 GA-treated and 18 untreated patients with MS was performed by flow cytometry; 6 GA-treated patients with MS were analyzed longitudinally. GA-mediated effects on B-cell antigen-presenting function were investigated in EAE, or, alternatively, B cells were treated with GA in vitro using vehicle as a control.Results In MS, GA diminished transitional B-cell and plasmablast frequency, downregulated CD69, CD25, and CD95 expression, and decreased TNF-α production, whereas IL-10 secretion and MHC Class II expression were increased. In EAE, we observed an equivalent dampening of proinflammatory B-cell properties and an enhanced expression of MHC Class II. When used as antigen-presenting cells for activation of naive T cells, GA-treated B cells promoted development of regulatory T cells, whereas proinflammatory T-cell differentiation was diminished.Conclusions GA immune modulates B-cell function in EAE and MS and efficiently interferes with pathogenic B cell–T cell interaction.APC=antigen-presenting cell; EAE=experimental autoimmune encephalomyelitis; GA=glatiramer acetate; NMO=neuromyelitis optica; PBMC=peripheral blood mononuclear cell; PBS=phosphate-buffered saline; RRMS=relapsing-remitting MS; WT=wild type