PT - JOURNAL ARTICLE AU - Anna García-Serra AU - Marija Radosevic AU - José Ríos AU - Esther Aguilar AU - Estibaliz Maudes AU - Jon Landa AU - Lidia Sabater AU - Eugenia Martinez-Hernandez AU - Jesús Planagumà AU - Josep Dalmau TI - Blocking Placental Class G Immunoglobulin Transfer Prevents NMDA Receptor Antibody Effects in Newborn Mice AID - 10.1212/NXI.0000000000001061 DP - 2021 Nov 01 TA - Neurology - Neuroimmunology Neuroinflammation PG - e1061 VI - 8 IP - 6 4099 - http://nn.neurology.org/content/8/6/e1061.short 4100 - http://nn.neurology.org/content/8/6/e1061.full SO - Neurol Neuroimmunol Neuroinflamm2021 Nov 01; 8 AB - Background and Objectives To determine in a mouse model whether neonatal Fc receptor (FcRn) blockade prevents the placental transfer of class G immunoglobulin (IgG) derived from patients with anti-NMDA receptor (NMDAR) encephalitis and their pathogenic effects on the fetuses and offspring.Methods Pregnant C57BL/6J mice were administered via tail vein FcRn antibody (FcRn-ab) or saline solution 6 hours before administration of patients' or controls' IgG on days 14, 15, and 16 of gestation. Three experimental groups were established: mice receiving controls' IgG, patients' IgG, or patients' IgG along with pretreatment with FcRn-ab. Immunohistochemical staining, confocal microscopy, hippocampal long-term potentiation, and standardized developmental and behavioral tasks were used to assess the efficacy of treatment with FcRn-ab.Results In pregnant mice that received patients' IgG, treatment with FcRn-ab prevented the IgG from reaching the fetal brain, abrogating the decrease of NMDAR clusters and the reduction of cortical plate thickness that were observed in fetuses from untreated pregnant mice. Moreover, among the offspring of mothers that received patients' IgG, those whose mothers were treated with FcRn-ab did not develop the alterations that occurred in offspring of untreated mothers, including impairment in hippocampal plasticity, delay in innate reflexes, and visuospatial memory deficits.Discussion FcRn blockade prevents placental transfer of IgG from patients with anti-NMDAR encephalitis and abrogates the synaptic and neurodevelopmental alterations caused by patients' antibodies. This model has potential therapeutic implications for other antibody-mediated diseases of the CNS during pregnancy.BBB=blood-brain barrier; CBA=cell-based assay; DAPI=6-diamidino-2-phenylindole dihydrochloride; Fc=fragment, crystallizable of IgG antibodies; FcRn=neonatal Fc receptor; FcRn-ab=FcRn antibody; fEPSP=field excitatory postsynaptic potential; FNIT=fetal and neonatal immune thrombocytopenia; GEE=generalized estimated equation; HEK=human embryonic kidney; IgG=class G immunoglobulin; LTP=long-term potentiation; NMDAR=NMDA receptor; NOL=novel object location; PD=postnatal day; PSD95=postsynaptic density protein 95; RT=room temperature