PT  - JOURNAL ARTICLE
AU  - Imke Metz
AU  - Tim Beißbarth
AU  - David Ellenberger
AU  - Florence Pache
AU  - Lidia Stork
AU  - Marius Ringelstein
AU  - Orhan Aktas
AU  - Sven Jarius
AU  - Brigitte Wildemann
AU  - Hassan Dihazi
AU  - Tim Friede
AU  - Wolfgang Brück
AU  - Klemens Ruprecht
AU  - Friedemann Paul
TI  - Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis
AID  - 10.1212/NXI.0000000000000204
DP  - 2016 Apr 01
TA  - Neurology - Neuroimmunology Neuroinflammation
PG  - e204
VI  - 3
IP  - 2
4099  - http://nn.neurology.org/content/3/2/e204.short
4100  - http://nn.neurology.org/content/3/2/e204.full
SO  - Neurol Neuroimmunol Neuroinflamm2016 Apr 01; 3
AB  - Objective: To assess in an observational study whether serum peptide antibody reactivities may distinguish aquaporin-4 (AQP4) antibody (Ab)–positive and -negative neuromyelitis optica spectrum disorders (NMOSD) and relapsing-remitting multiple sclerosis (RRMS).Methods: We screened 8,700 peptides that included human and viral antigens of potential relevance for inflammatory demyelinating diseases and random peptides with pooled sera from different patient groups and healthy controls to set up a customized microarray with 700 peptides. With this microarray, we tested sera from 66 patients with AQP4-Ab-positive (n = 16) and AQP4-Ab-negative (n = 19) NMOSD, RRMS (n = 11), and healthy controls (n = 20).Results: Differential peptide reactivities distinguished NMOSD subgroups from RRMS in 80% of patients. However, the 2 NMOSD subgroups were not well-discriminated, although those patients are clearly separated by their antibody reactivities against AQP4 in cell-based assays. Elevated reactivities to myelin and Epstein-Barr virus peptides were present in RRMS and to AQP4 and AQP1 peptides in AQP4-Ab-positive NMOSD.Conclusions: While AQP4-Ab-positive and -negative NMOSD subgroups are not well-discriminated by peptide antibody reactivities, our findings suggest that peptide antibody reactivities may have the potential to distinguish between both NMOSD subgroups and MS. Future studies should thus concentrate on evaluating peptide antibody reactivities for the differentiation of AQP4-Ab-negative NMOSD and MS.AQP4-Ab=aquaporin-4 antibody; EBNA-1=Epstein-Barr nuclear antigen-1; EBV=Epstein-Barr virus; IgG=immunoglobulin G; MOG=myelin oligodendrocyte glycoprotein; MS=multiple sclerosis; NMO=neuromyelitis optica; NMOSD=neuromyelitis optica spectrum disorders; RRMS=relapsing-remitting multiple sclerosis