RT Journal Article SR Electronic T1 Proinflammatory B-cell profile in the early phases of MS predicts an active disease JF Neurology - Neuroimmunology Neuroinflammation JO Neurol Neuroimmunol Neuroinflamm FD Lippincott Williams & Wilkins SP e431 DO 10.1212/NXI.0000000000000431 VO 5 IS 2 A1 Guerrier, Thomas A1 Labalette, Myriam A1 Launay, David A1 Lee-Chang, Catalina A1 Outteryck, Olivier A1 Lefèvre, Guillaume A1 Vermersch, Patrick A1 Dubucquoi, Sylvain A1 Zéphir, Hélène YR 2018 UL http://nn.neurology.org/content/5/2/e431.abstract AB Objective: To assess whether any alteration of B-cell subset distribution and/or the cytokine production capacities of B cells could be associated with any stage of MS and could be predictive of MS evolution.Methods: We prospectively enrolled radiologically isolated syndrome (RIS), clinically isolated syndrome (CIS), naive patients with relapsing remitting MS (RRMS) of any disease modifying drug, and healthy controls (HCs). Peripheral blood B-cell subset distributions and the interleukin (IL)-6/IL-10–producing B-cell ratio were assessed by flow cytometry to evaluate their proinflammatory and anti-inflammatory functional properties.Results: Twelve RIS, 46 CIS, 31 RRMS patients, and 36 HCs were enrolled. We observed that a high IL-6/IL-10–producing B-cell ratio in patients with RIS/CIS was associated with the evolution of the disease in the short term (6 months). This imbalance in cytokine production was mainly explained by an alteration of the production of IL-10 by B cells, especially for the transitional B-cell subset. In addition, a significant increase in IgD−/CD27− B cells was detected in patients with CIS and RRMS compared with HCs (p = 0.01). Apart from this increase in exhausted B cells, no other variation in B-cell subsets was observed.Conclusions: The association between a high IL-6/IL-10–producing B-cell ratio and the evolution of patients with RIS/CIS suggest a skew of B cells toward proinflammatory properties that might be implicated in the early phases of MS disease.CIS=clinically isolated syndrome; EAE=experimental autoimmune encephalomyelitis; GM-CSF=granulocyte-macrophage colony-stimulating factor; HC=healthy control; IL=interleukin; LT=lymphotoxin; PBMC=peripheral blood mononuclear cell; RIS=radiologically isolated syndrome; ROC=receiver operating characteristic; RRMS=relapsing remitting MS; TNFα=tumor necrosis factor–alpha