RT Journal Article
SR Electronic
T1 Varied antibody reactivities and clinical relevance in anti-GQ1b antibody–related diseases
JF Neurology - Neuroimmunology Neuroinflammation
JO Neurol Neuroimmunol Neuroinflamm
FD Lippincott Williams & Wilkins
SP e501
DO 10.1212/NXI.0000000000000501
VO 5
IS 6
A1 Yoshikawa, Keisuke
A1 Kuwahara, Motoi
A1 Morikawa, Miyuki
A1 Fukumoto, Yuta
A1 Yamana, Masaki
A1 Yamagishi, Yuko
A1 Kusunoki, Susumu
YR 2018
UL http://nn.neurology.org/content/5/6/e501.abstract
AB Objective To investigate the relationship between antibody reactivities against glycolipid complexes and clinical features in Miller Fisher syndrome (MFS), Bickerstaff brainstem encephalitis (BBE), and Guillain-Barré syndrome with ophthalmoplegia (GBS-OP).Methods Using glycoarray, antibodies against 10 glycolipid antigens (GM1, GM2, GM4, GD1a, GD1b, GQ1b, galactocerebroside, lactosylceramide, GA1, and sulfatide) and 45 glycolipid complexes consisting 2 of the glycolipids were examined in the sera of 63 patients with GBS-OP, 37 patients with MFS, and 27 patients with BBE.Results Antibodies to antigens containing GQ1b were identified in 73% of patients with GBS-OP (46/63), 86.5% of patients with MFS (32/37), and 74.1% of patients with BBE (20/27), and GD1b-related antibodies were identified in 49.2% of patients with GBS-OP (31/63), 29.7% of patients with MFS (11/37), and 11.1% of patients with BBE (3/27). Comparing clinical features between patients with GBS-OP with and without both antibodies, the proportion of patients requiring artificial ventilation and presenting moderate or severe muscle weakness was higher in the positive group than in the negative group (p = 0.017 and p = 0.046, respectively).Conclusions Antibodies binding to antigens containing GD1b and to those containing GQ1b may be involved in the development of limb weakness and respiratory failure in anti-GQ1b antibody–related diseases.BBE=Bickerstaff brainstem encephalitis; BSA=bovine serum albumin; GBS=Guillain-Barré syndrome; IgM=immunoglobulin M; MFS=Miller Fisher syndrome; OP=ophthalmoplegia; PBS=phosphate-buffered saline; TLC=thin-layer chromatography