PT - JOURNAL ARTICLE AU - Singhal, Tarun AU - O'Connor, Kelsey AU - Dubey, Shipra AU - Pan, Hong AU - Chu, Renxin AU - Hurwitz, Shelley AU - Cicero, Steven AU - Tauhid, Shahamat AU - Silbersweig, David AU - Stern, Emily AU - Kijewski, Marie AU - DiCarli, Marcelo AU - Weiner, Howard L. AU - Bakshi, Rohit TI - Gray matter microglial activation in relapsing vs progressive MS AID - 10.1212/NXI.0000000000000587 DP - 2019 Sep 01 TA - Neurology - Neuroimmunology Neuroinflammation PG - e587 VI - 6 IP - 5 4099 - http://nn.neurology.org/content/6/5/e587.short 4100 - http://nn.neurology.org/content/6/5/e587.full SO - Neurol Neuroimmunol Neuroinflamm2019 Sep 01; 6 AB - Objective To determine the value of [F-18]PBR06-PET for assessment of microglial activation in the cerebral gray matter in patients with MS.Methods Twelve patients with MS (7 relapsing-remitting and 5 secondary progressive [SP]) and 5 healthy controls (HCs) had standardized uptake value (SUV) PET maps coregistered to 3T MRI and segmented into cortical and subcortical gray matter regions. SUV ratios (SUVRs) were global brain normalized. Voxel-by-voxel analysis was performed using statistical parametric mapping (SPM). Normalized brain parenchymal volumes (BPVs) were determined from MRI using SIENAX.Results Cortical SUVRs were higher in the hippocampus, amygdala, midcingulate, posterior cingulate, and rolandic operculum and lower in the medial-superior frontal gyrus and cuneus in the MS vs HC group (all p < 0.05). Subcortical gray matter SUVR was higher in SPMS vs RRMS (+10.8%, p = 0.002) and HC (+11.3%, p = 0.055) groups. In the MS group, subcortical gray matter SUVR correlated with the Expanded Disability Status Scale (EDSS) score (r = 0.75, p = 0.005) and timed 25-foot walk (T25FW) (r = 0.70, p = 0.01). Thalamic SUVRs increased with increasing EDSS scores (r = 0.83, p = 0.0008) and T25FW (r = 0.65, p = 0.02) and with decreasing BPV (r = −0.63, p = 0.03). Putaminal SUVRs increased with increasing EDSS scores (0.71, p = 0.009) and with decreasing BPV (r = −0.67, p = 0.01). On SPM analysis, peak correlations of thalamic voxels with BPV were seen in the pulvinar and with the EDSS score and T25FW in the dorsomedial thalamic nuclei.Conclusions This study suggests that [F-18]PBR06-PET detects widespread abnormal microglial activation in the cerebral gray matter in MS. Increased translocator protein binding in subcortical gray matter regions is associated with brain atrophy and may link to progressive MS.AAL=automated anatomic labeling; BPV=brain parenchymal volume; C-SUVR=total cortical gray standardized uptake value ratio; EDSS=Expanded Disability Status Scale; HC=healthy control; RCP=radiochemical purity; ROI=region of interest; RR=relapsing-remitting; SC-SUVR=total subcortical gray matter standardized uptake value ratio; SP=secondary progressive; SUVR=standardized uptake value ratio; T25FW=timed 25-foot walk; TSPO=translocator protein